FBXO6 Monoklonaler Antikörper

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• KD/KO Validated

FBXO6 Monoklonal Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

3B7F9

Kat-Nr. : 67697-1-PBS

Synonyme

3B7F9, F box only protein 6, F box protein 6, FBG2, F-box only protein 6

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  WB analysis using 67697-1-Ig (same clone as 67697-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67697-1-Ig (FBXO6 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67697-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 67697-1-Ig (same clone as 67697-1-PBS)

  WB result of FBXO6 antibody (67697-1-Ig; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FBXO6 transfected Jurkat cells. This data was developed using the same antibody clone with 67697-1-PBS in a different storage buffer formulation.

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  IF Staining of HEK-293 using 67697-1-Ig (same clone as 67697-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using FBXO6 antibody (67697-1-Ig, Clone: 3B7F9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67697-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

67697-1-PBS bindet in WB, IF/ICC, Indirect ELISA FBXO6 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: FBXO6 fusion protein Ag30512
• Vollständiger Name: F-box protein 6
• Berechnetes Molekulargewicht: 293 aa, 34 kDa
• Beobachtetes Molekulargewicht: 34 kDa
• GenBank-Zugangsnummer: BC020880
• Gene symbol: FBXO6
• Gene ID (NCBI): 26270
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

FBXO6(F-box only protein 6 ) is also named as FBG2, FBS2, FBX6 and belongs to the F-box protein family. FBXO6 is the substrate recognition component of a Skp1-Cullin1-F-box protein (SCF) ubiquitin E3 ligase complex, recognizing the chitobiose in unfolded N-glycoprotein to target glycoproteins for polyubiquitination and degradation. It can regulate Chk1 ubiquitination and degradation in both normally cycling cells and during replication stress. This protein is mainly detected in the cytoplasm in both cultured cells and in tumor tissues. Its expression is a predictive biomarker of tumor responsiveness to the important anticancer drugs. Positive Fbxo6 expression correlated with early TNM stage and favorable prognosis of NSCLC patients. (PMID:19716789, PMID: 27855403, PMID: 31140586).