FBXW11 Polyklonaler Antikörper

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FBXW11 Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 13149-1-PBS

Synonyme

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  WB analysis using 13149-1-AP (same clone as 13149-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 13149-1-AP (FBXW11 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13149-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 13149-1-AP (same clone as 13149-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 13149-1-AP (FBXW11 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13149-1-PBS in a different storage buffer formulation.

• 

  WB analysis of SGC-7901 using 13149-1-AP (same clone as 13149-1-PBS)

  SGC-7901 cells were subjected to SDS PAGE followed by western blot with 13149-1-AP (FBXW11 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13149-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 13149-1-AP (same clone as 13149-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 13149-1-AP (FBXW11 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13149-1-PBS in a different storage buffer formulation.

• 

  WB analysis of SGC-7901 using 13149-1-AP (same clone as 13149-1-PBS)

  SGC-7901 cells were subjected to SDS PAGE followed by western blot with 13149-1-AP (FBXW11 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13149-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human stomach using 13149-1-AP (same clone as 13149-1-PBS)

  human stomach tissue were subjected to SDS PAGE followed by western blot with 13149-1-AP (FBXW11 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13149-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 13149-1-AP (same clone as 13149-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 13149-1-AP (FBXW11 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13149-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human stomach cancer using 13149-1-AP (same clone as 13149-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 13149-1-AP (FBXW11 Antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 13149-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human stomach cancer using 13149-1-AP (same clone as 13149-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 13149-1-AP (FBXW11 Antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 13149-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HCT 116 using 13149-1-AP (same clone as 13149-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HCT 116 cells using FBXW11 antibody (13149-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 13149-1-PBS in a different storage buffer formulation.

• 

  IF Staining of A431 using 13149-1-AP (same clone as 13149-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using FBXW11 antibody (13149-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 13149-1-PBS in a different storage buffer formulation.

• 

  IF Staining of U2OS using 13149-1-AP (same clone as 13149-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using FBXW11 antibody (13149-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 13149-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

13149-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA FBXW11 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: FBXW11 fusion protein Ag3761
• Vollständiger Name: F-box and WD repeat domain containing 11
• Berechnetes Molekulargewicht: 542 aa, 61 kDa
• Beobachtetes Molekulargewicht: 61 kDa
• GenBank-Zugangsnummer: BC026213
• Gene symbol: FBXW11
• Gene ID (NCBI): 23291
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

FBXW11 (also known as HOS or β-TrCP2) is a member of F-box family proteins and plays critical role in regulating the ubiquitination of phosphorylated substrates. Abnormal expression of several FBXW11 is involved in the modulation of various biological events, such as cell cycle, differentiation, migration, inflammation, and apoptosis, through targeting multiple different substrates. For instance, FBXW11 could bind to the phosphorylated IκB and β-catenin, promoting their degradation via the ubiquitin-proteasome system. In addition, FBXW11 expression is markedly increased in mouse skin tumors and promotes tumor growth by activating the NF-κB signaling (PMID: 33640602). FBXW11 has 3 isoforms with the molecular mass of 58-62 kDa.