FBXW2 Polyklonaler Antikörper

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• KD/KO Validated

FBXW2 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(8)

Kat-Nr. : 11499-1-AP

Synonyme

F-box and WD-40 domain-containing protein 2, F-box/WD repeat-containing protein 2, FBW2, FBXW 2, FWD2

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  WB analysis of HEK-293 using 11499-1-AP

  WB result of FBXW2 antibody (11499-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-FBXW2 transfected HEK-293 cells.

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  WB analysis of HEK-293 using 11499-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11499-1-AP (FBXW2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 11499-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 11499-1-AP (FBXW2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 11499-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11499-1-AP (FBXW2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, Mausnierengewebe
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 5 publications below
• WB: See 7 publications below
• IHC: See 2 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Produktinformation

11499-1-AP bindet in WB, IHC, IF, IP, ELISA FBXW2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: FBXW2 fusion protein Ag2045
• Vollständiger Name: F-box and WD repeat domain containing 2
• Berechnetes Molekulargewicht: 44 kDa, 52 kDa
• Beobachtetes Molekulargewicht: 43 kDa
• GenBank-Zugangsnummer: BC018738
• Gene symbol: FBXW2
• Gene ID (NCBI): 26190
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The human FBW2 polypeptide contains an N-terminal F-box motif and a C-terminal domain of five WD repeats. A WD repeat is a motif typically consisting of 44-60 amino acids with a signature tryptophan and aspartic acid dipeptide at its C-terminus. The WD repeats in FBW2 recognize GCM1 in a way that is facilitated by GSK3b. Two isoforms were produced by alternative splicing with predicted MW of 52 and 43 kDa according to UniProt, and Catalog#11499-1-AP can recognise both.

Publikationen

Species	Application	Title
human	WB	Nat Commun; The β-TrCP-FBXW2-SKP2 axis regulates lung cancer cell growth with FBXW2 acting as a tumour suppressor.; Authors - Jie Xu ; KD Validated
human	WB	Cancer Lett; FBXO43 promotes cell cycle progression in cancer cells through stabilizing SKP2; Authors - Liyun Zheng
human	WB	Cell Mol Gastroenterol Hepatol; TAK1 Is a Novel Target in Hepatocellular Carcinoma and Contributes to Sorafenib Resistance.; Authors - Shunjie Xia ; KD Validated
human	WB	Cell Mol Life Sci; FBXW2 inhibits prostate cancer proliferation and metastasis via promoting EGFR ubiquitylation and degradation.; Authors - Tao Zhou ; KD Validated
human	IP	J Biol Chem; USP2 is an SKP2 deubiquitylase that stabilizes both SKP2 and its substrates.; Authors - Fengwu Zhang
human	WB,IHC	Nat Commun; FBXW2 suppresses migration and invasion of lung cancer cells via promoting β-catenin ubiquitylation and degradation.; Authors - Fei Yang ; KD Validated