FBXW4 Polyklonaler Antikörper

FBXW4 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Rezensionen (1)

Kat-Nr. : 10657-1-AP

Synonyme

DAC, Dactylin, FBW4, FBWD4, FBXW4, SHFM3, SHSF3

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  WB analysis of mouse lung using 10657-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 10657-1-AP (FBXW4 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 10657-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 10657-1-AP (FBXW4 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human pancreas using 10657-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 10657-1-AP (FBXW4 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human pancreas using 10657-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 10657-1-AP (FBXW4 Antibody) at dilution of 1:200 (under 10x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mauslungengewebe, Mauslebergewebe
• Erfolgreiche Detektion in IHC: humanes Pankreasgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

10657-1-AP bindet in WB, IHC, ELISA FBXW4 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: FBXW4 fusion protein Ag1047
• Vollständiger Name: F-box and WD repeat domain containing 4
• Berechnetes Molekulargewicht: 46 kDa
• Beobachtetes Molekulargewicht: 50 kDa
• GenBank-Zugangsnummer: BC007380
• Gene symbol: FBXW4
• Gene ID (NCBI): 6468
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

F-box proteins have been shown to be critical for the ubiquitin-mediated degradation of cellular regulatory proteins, and they are a family of eukaryotic proteins characterized by an approximately 40 amino acid motif. SCF complex, a class of ubiquitin ligases, consists of invariable components, Skp1 and Cullin, and variable components of F-box proteins, which have a primary role in determining substrate specificity. FBXW4, also known as SHFM3, encodes F-box and WD-40 domain-containing protein 4. Defects in SHFM3 are a cause of split-hand/foot malformation type 3 (SHFM3), an autosomal dominant disorder characterized by hypoplasia/aplasia of the central digits with fusion of the remaining digits.

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

wei (Verified Customer) (07-23-2025)

USED WORK WELL

• Applications: Immunohistochemistry, Flow Cytometry