FBXW5 Polyklonaler Antikörper

FBXW5 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

1

Kat-Nr. : 18321-1-AP

Synonyme

Fbw5, FBXW5, RP11 229P13.10

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Galerie der Validierungsdaten

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  WB analysis using 18321-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18321-1-AP (FBXW5 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse testis using 18321-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 18321-1-AP (FBXW5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 18321-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 18321-1-AP (FBXW5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 18321-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18321-1-AP (FBXW5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 18321-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18321-1-AP (FBXW5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, K-562-Zellen
• Erfolgreiche Detektion in IHC: Maushodengewebe, humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

18321-1-AP bindet in WB, IHC, ELISA FBXW5 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: FBXW5 fusion protein Ag13163
• Vollständiger Name: F-box and WD repeat domain containing 5
• Berechnetes Molekulargewicht: 64 kDa
• Beobachtetes Molekulargewicht: 65 kDa
• GenBank-Zugangsnummer: BC014297
• Gene symbol: FBXW5
• Gene ID (NCBI): 54461
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

F-box proteins have been shown to be critical for the ubiquitin-mediated degradation of cellular regulatory proteins, and they are a family of eukaryotic proteins characterized by an approximately 40 amino acid motif. SCF complex, a class of ubiquitin ligases, consists of invariable components, Skp1 and Cullin, and variable components of F-box proteins, which have a primary role in determining substrate specificity. FBXW5 (F-box/WD repeat-containing protein 5) is a novel protein containing WD-40 repeats, which were frequently involved in protein-protein interactions. FBXW5 negatively regulates TAK1 in the IL-1beta signaling pathway. By forming different E3 ubiquitin-protein ligase complexes, FBXW5 mediates the polyubiquitination and subsequent degradation of TSC2 and centriolar protein HsSAS-6.

Publikationen

Species	Application	Title
human	WB	J Cell Physiol; The Ubiquitin Ligase SCF(FBXW7α) Promotes ATA3 Degradation.; Authors - Nan Song