FBXW7 Polyklonaler Antikörper

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• KD/KO Validated

FBXW7 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, IF, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(41)

Kat-Nr. : 28424-1-AP

Synonyme

AGO, Archipelago homolog, CDC4, DKFZp686F23254, F box protein FBX30, FBW6, FBW7, FBX30, FBXO30, FBXW6, FBXW7, FLJ16457, hAgo, hCdc4, SEL 10, SEL10

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  WB analysis using 28424-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28424-1-AP (FBXW7 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 28424-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 28424-1-AP (FBXW7 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 28424-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 28424-1-AP (FBXW7 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 28424-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 28424-1-AP (FBXW7 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 28424-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 28424-1-AP (FBXW7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HepG2-Zellen, MCF-7-Zellen, Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe, humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 10 publications below
• WB: See 38 publications below
• IHC: See 9 publications below
• IF: See 5 publications below
• IP: See 7 publications below

Produktinformation

28424-1-AP bindet in WB, IHC, IF, IP, ELISA FBXW7 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: FBXW7 fusion protein Ag28471
• Vollständiger Name: F-box and WD repeat domain containing 7
• Berechnetes Molekulargewicht: 66 kDa
• Beobachtetes Molekulargewicht: 100-110 kDa, 66-75 kDa
• GenBank-Zugangsnummer: NM_001349798
• Gene symbol: FBXW7
• Gene ID (NCBI): 55294
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

FBXW7, also named as FBW7, FBX30, SEL10 and hAgo, is a substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. It probably recognizes and binds to phosphorylated target proteins. FBXW7 is involved in the degradation of cyclin-E, MYC, NOTCH1 released notch intracellular domain (NICD), and probably PSEN1. FBXW7 is a general tumor suppressor in human cancer (PMID: 17909001). FBXW7 has 3 isoforms (α/β/γ) with the calculated molecular mass of 80 kDa, 70 kDa and 66 kDa, and apparent molecular mass of 100-110 kDa and 66-75 kDa (PMID: 31346036/PMID: 22864569). K48 autoubiquitination is consistent with maintenance of ubiquitinated FBW7 (Ub-FBW7, 200 kDa)(PMID: 32353058).

Publikationen

Species	Application	Title
human	WB	Mol Cancer; Loss of FBXW7-mediated degradation of BRAF elicits resistance to BET inhibitors in adult T cell leukemia cells.; Authors - Chien-Hung Yeh
human	WB,IP	J Exp Med; Somatic gain-of-function mutations in BUD13 promote oncogenesis by disrupting Fbw7 function; Authors - Jianfeng Chen
mouse,rat	WB,IF,IP	Dev Cell; Nuclear import carrier Hikeshi cooperates with HSP70 to promote murine oligodendrocyte differentiation and CNS myelination; Authors - Li Li ; KD Validated
human	WB,IHC,IF	Cancer Lett; Inhibition of KIF20A enhances the immunotherapeutic effect of hepatocellular carcinoma by enhancing c-Myc ubiquitination; Authors - Shujia Chen
mouse	WB	Theranostics; Histone demethylase JMJD3 protects against renal fibrosis by suppressing TGFβ and Notch signaling and preserving PTEN expression.; Authors - Chao Yu
mouse	WB	EMBO Rep; The 18S rRNA m6 A methyltransferase METTL5 promotes mouse embryonic stem cell differentiation.; Authors - Ming Xing