FCER1G Rekombinanter Antikörper

FCER1G Rekombinant Antikörper für IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

242829C5

Kat-Nr. : 85390-1-RR

Synonyme

242829C5, Fc epsilon RI gamma, Fc receptor gamma-chain, Fc-epsilon RI-gamma, FceRI gamma

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  IF Staining of THP-1 using 85390-1-RR

  Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using FCER1G antibody (85390-1-RR, Clone: 242829C5 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  IF Staining of RAW 264.7 using 85390-1-RR

  Immunofluorescent analysis of (4% PFA) fixed RAW 264.7 cells using FCER1G antibody (85390-1-RR, Clone: 242829C5 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 85390-1-RR

  Biolayer interferometry (BLl) kinetic assays of 85390-1-RR against Human FCER1G were performed. The affinity constant is 2.23 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF/ICC: THP-1-Zellen, RAW 264.7-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

85390-1-RR bindet in IF/ICC, ELISA FCER1G und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: FCER1G fusion protein Ag4442
• Vollständiger Name: Fc fragment of IgE, high affinity I, receptor for; gamma polypeptide
• Berechnetes Molekulargewicht: 87 aa, 10 kDa
• GenBank-Zugangsnummer: BC033872
• Gene symbol: FcRγ
• Gene ID (NCBI): 2207
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

FCER1G (Fc receptor gamma-chain, FcRgamma), also known as Fc-epsilon RI-gamma, or IgE Fc receptor subunit gamma (FceRI gamma), is a component of the high-affinity immunoglobulin E (IgE) receptor (Fc epsilon RI) which is a tetrameric complex of one alpha subunit, one beta subunit, and two disulfide-linked gamma subunits (PMID: 2146219; 2531187). Fc epsilon RI is present exclusively on mast cells and basophils, mediating allergic inflammatory signaling (PMID: 17438574). FCER1G is widely expressed and contains an immunoreceptor tyrosine-based activation motif (ITAM) that transduces activation signals from various immunoreceptors (PMID: 19098920).