FCER1G Rekombinanter Antikörper

FCER1G Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Hausschwein, human, Maus

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

242829E3

Kat-Nr. : 85390-2-RR

Synonyme

242829E3, Fc epsilon RI gamma, Fc receptor gamma-chain, Fc-epsilon RI-gamma, FceRI gamma

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  WB analysis using 85390-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85390-2-RR (FCER1G antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 85390-2-RR

  Biolayer interferometry (BLl) kinetic assays of 85390-2-RR against Human FCER1G were performed. The affinity constant is 1.16 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: THP-1-Zellen, HL-60-Zellen, Hausschwein-Milzgewebe, RAW 264.7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

85390-2-RR bindet in WB, ELISA FCER1G und zeigt Reaktivität mit Hausschwein, human, Maus

• Getestete Reaktivität: Hausschwein, human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: FCER1G fusion protein Ag4442
• Vollständiger Name: Fc fragment of IgE, high affinity I, receptor for; gamma polypeptide
• Berechnetes Molekulargewicht: 87 aa, 10 kDa
• Beobachtetes Molekulargewicht: 10 kDa
• GenBank-Zugangsnummer: BC033872
• Gene symbol: FcRγ
• Gene ID (NCBI): 2207
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

FCER1G (Fc receptor gamma-chain, FcRgamma), also known as Fc-epsilon RI-gamma, or IgE Fc receptor subunit gamma (FceRI gamma), is a component of the high-affinity immunoglobulin E (IgE) receptor (Fc epsilon RI) which is a tetrameric complex of one alpha subunit, one beta subunit, and two disulfide-linked gamma subunits (PMID: 2146219; 2531187). Fc epsilon RI is present exclusively on mast cells and basophils, mediating allergic inflammatory signaling (PMID: 17438574). FCER1G is widely expressed and contains an immunoreceptor tyrosine-based activation motif (ITAM) that transduces activation signals from various immunoreceptors (PMID: 19098920).