CD64 Polyklonaler Antikörper

CD64 Polyklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Kat-Nr. : 27563-1-AP

Synonyme

CD64A, Fc gamma RI, Fc gamma RIA, FCG1, Fc-gamma RI

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  WB analysis using 27563-1-AP

  THP-1 cells were subjected to SDS PAGE followed by western blot with 27563-1-AP (CD64 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of U-937 using 27563-1-AP

  U-937 cells were subjected to SDS PAGE followed by western blot with 27563-1-AP (CD64 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HL-60 using 27563-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 27563-1-AP (CD64 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IF Staining of THP-1 using 27563-1-AP

  Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using CD64 antibody (27563-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: THP-1-Zellen, HL-60-Zellen, U-937-Zellen
• Erfolgreiche Detektion in IF/ICC: THP-1-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

27563-1-AP bindet in WB, IF/ICC, ELISA CD64 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CD64 fusion protein Ag26727
• Vollständiger Name: Fc fragment of IgG, high affinity Ia, receptor (CD64)
• Berechnetes Molekulargewicht: 374 aa, 43 kDa
• Beobachtetes Molekulargewicht: 65-70 kDa, 43-45 kDa
• GenBank-Zugangsnummer: BC032634
• Gene symbol: FCGR1A
• Gene ID (NCBI): 2209
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Fcγ receptor comprise a multigene family of integral membrane glycoproteins that exhibit complex activation or inhibitory effects on cell functions after aggregation by complexed immunoglobulin G (IgG) (PMID: 17005690 ). CD64, also known as FcγRIA, is a high affinity receptor for the Fc region of IgG. It is expressed by monocytes/macrophages, activated neutrophils, dendritic cells, and early myeloid cells (PMID: 23293080; 19642859; 7680917). CD64 functions in both innate and adaptive immune responses. The calculated molecular weight of CD64 is 43 kDa, while the glycosylated CD64 has a higher apparent molecular weight.

Publikationen

Species	Application	Title
human	WB	Front Oncol; Cancer Cell-Derived Exosomes Promote HCC Tumorigenesis Through Hedgehog Pathway.; Authors - Li Li