FCGR2B / CD32b Polyklonaler Antikörper

FCGR2B / CD32b Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Kat-Nr. : 21541-1-AP

Synonyme

FCGR2B, Fc-gamma RII-b, FCG2, Fc gamma RIIb, Fc gamma RII b

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  WB analysis of human placenta using 21541-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 21541-1-AP (FCGR2B / CD32b antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 21541-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 21541-1-AP (FCGR2B / CD32b antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 21541-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 21541-1-AP (FCGR2B / CD32b antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 21541-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 21541-1-AP (FCGR2B / CD32b antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 21541-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 21541-1-AP (FCGR2B / CD32b antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human placenta using 21541-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 21541-1-AP (FCGR2B / CD32b antibody at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human spleen using 21541-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 21541-1-AP (FCGR2B / CD32b antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human spleen using 21541-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 21541-1-AP (FCGR2B / CD32b antibody at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human tonsillitis using 21541-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 21541-1-AP (FCGR2B / CD32b antibody at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Plazenta-Gewebe, Raji-Zellen
• Erfolgreiche Detektion in IHC: humanes Tonsillitisgewebe, humanes Plazenta-Gewebe, humanes Milzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunhistochemie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

21541-1-AP bindet in WB, IHC, ELISA FCGR2B / CD32b und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: FCGR2B / CD32b fusion protein Ag16189
• Vollständiger Name: Fc fragment of IgG, low affinity IIb, receptor (CD32)
• Berechnetes Molekulargewicht: 310 aa, 34 kDa
• Beobachtetes Molekulargewicht: 36 kDa
• GenBank-Zugangsnummer: BC031992
• Gene symbol: CD32b
• Gene ID (NCBI): 2213
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
		Mol Ther; High-dose systemic adeno-associated virus vector administration causes liver and sinusoidal endothelial cell injury; Authors - Juliette Hordeaux