• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

FCGR3A/CD16a Polyklonaler Antikörper

FCGR3A/CD16a Polyklonal Antikörper für WB, IF-P, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF-P, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 30356-1-PBS

Synonyme

CD16, Fc gamma RIII alpha, Fc gamma RIII, CD16-II, CD16a antigen

Filter:
  • Western Blot (WB) analysis of various lysates using FCGR3A/CD16a Polyclonal antibody (30356-1-AP)

    WB analysis using 30356-1-AP (same clone as 30356-1-PBS)

    HL-60 cells were subjected to SDS PAGE followed by western blot with 30356-1-AP (FCGR3A/CD16a antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30356-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using FCGR3A/CD16a Polyclonal antibody (30356-1-AP)

    WB analysis using 30356-1-AP (same clone as 30356-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 30356-1-AP (FCGR3A/CD16a antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30356-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse spleen tissue using FCGR3A/CD16a Polyclonal antibody (30356-1-AP)

    IF Staining of mouse spleen using 30356-1-AP (same clone as 30356-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using FCGR3A/CD16a antibody (30356-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30356-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

30356-1-PBS bindet in WB, IF-P, Indirect ELISA FCGR3A/CD16a und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen FCGR3A/CD16a fusion protein Ag1281
Vollständiger Name Fc fragment of IgG, low affinity IIIa, receptor (CD16a)
Berechnetes Molekulargewicht 29 kDa
Beobachtetes Molekulargewicht65-70 kDa
GenBank-ZugangsnummerBC036723
Gene symbol CD16a
Gene ID (NCBI) 2214
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD16 is a low affinity Fc receptor. It has been identified as Fc receptors FcγRIIIa (CD16a) and FcγRIIIb (CD16b), encoded by two nearly identical genes, FCGR3A and the FCGR3B. In humans, FcγRIIIa/CD16a is a type I transmembrane receptor expressed on macrophages and NK cells. FcγRIIIb/CD16b is a GPI-linked Fc-receptor highly expressed on neutrophils. The two proteins differ by only four amino acid residues in the mature proteins(PMID: 30361439). FCGR3A has a calculated molecular weight of 29 kDa, with an apparent molecular weight of 50-80 kDa due to extensive glycosylation (PMID: 30655272; PMID: 27914599). The antibody is specific for FCGR3A/CD16a.