FCRLA Polyklonaler Antikörper

FCRLA Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Kat-Nr. : 26949-1-AP

Synonyme

FCRL, Fc receptor-related protein X, Fc receptor-like protein, Fc receptor-like and mucin-like protein 1, Fc receptor-like A

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  WB analysis of Raji using 26949-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 26949-1-AP (FCRLA Antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 26949-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 26949-1-AP (FCRLA Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of Daudi using 26949-1-AP

  Daudi cells were subjected to SDS PAGE followed by western blot with 26949-1-AP (FCRLA Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of Daudi using 26949-1-AP

  Daudi cells were subjected to SDS PAGE followed by western blot with 26949-1-AP (FCRLA Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 26949-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 26949-1-AP (FCRLA antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 26949-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 26949-1-AP (FCRLA antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Raji-Zellen, Daudi cells
• Erfolgreiche Detektion in IHC: humanes Tonsillitisgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:16000
• Immunhistochemie (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IHC: See 1 publications below

Produktinformation

26949-1-AP bindet in WB, IHC, ELISA FCRLA und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: FCRLA fusion protein Ag25331
• Vollständiger Name: Fc receptor-like A
• Berechnetes Molekulargewicht: 39 kDa
• Beobachtetes Molekulargewicht: 39-43 kDa
• GenBank-Zugangsnummer: BC006521
• Gene symbol: FCRLA
• Gene ID (NCBI): 84824
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	IHC	Int J Cancer; Aggressive characteristics of tumor deposits in colorectal cancer highlight the need for staging refinement in patients with 0-3 metastatic lymph nodes; Authors - Xi'e Hu