FDX1 Polyklonaler Antikörper

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• KD/KO Validated

FDX1 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

36

Kat-Nr. : 12592-1-AP

Synonyme

Adrenal ferredoxin, Adrenodoxin, mitochondrial, ADX, FDX, FDX1, ferredoxin 1, Hepatoredoxin, LOH11CR1D

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Galerie der Validierungsdaten

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  WB analysis using 12592-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12592-1-AP (FDX1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 12592-1-AP

  WB result of FDX1 antibody (12592-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FDX1 transfected HEK-293 cells.

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  WB analysis of HepG2 using 12592-1-AP

  WB result of FDX1 antibody (12592-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FDX1 transfected HepG2 cells.

• 

  WB analysis using 12592-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12592-1-AP (FDX1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 12592-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12592-1-AP (FDX1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 12592-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12592-1-AP (FDX1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 12592-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 12592-1-AP (FDX1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 12592-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 12592-1-AP (FDX1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 12592-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 12592-1-AP (FDX1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 12592-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 12592-1-AP (FDX1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 12592-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using FDX1 antibody (12592-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, HEK-293-Zellen, HepG2-Zellen, Maushodengewebe, PC-3-Zellen
• Erfolgreiche Detektion in IHC: humanes Nierengewebe, humanes Lebergewebe, humanes Ovarialkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 28 publications below
• IHC: See 11 publications below
• IF: See 5 publications below

Produktinformation

12592-1-AP bindet in WB, IHC, IF, ELISA FDX1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: FDX1 fusion protein Ag3301
• Vollständiger Name: ferredoxin 1
• Berechnetes Molekulargewicht: 184 aa, 19 kDa
• Beobachtetes Molekulargewicht: 14 kDa
• GenBank-Zugangsnummer: BC017063
• Gene symbol: FDX1
• Gene ID (NCBI): 2230
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

FDX1(Ferredoxin-1) is also named as ADX, FDX, YAH1, LOH11CR1D and belongs to the adrenodoxin/putidaredoxin family. It is a small iron-sulfur protein that transfers electrons from NADPH through ferredoxin reductase to a terminal cytochrome P450 and it can only reduce mitochondrial CYP enzymes that are essential in adrenal steroidogenesis, bile acid formation, and vitamin D synthesis. The full length has a transit peptide of 60 amino acids.

Publikationen

Species	Application	Title
human	WB	Nature; SLC25A39 is necessary for mitochondrial glutathione import in mammalian cells.; Authors - Ying Wang
human	WB	Mol Cell; Maintaining Iron Homeostasis Is the Key Role of Lysosomal Acidity for Cell Proliferation.; Authors - Ross A Weber
human	WB	Nat Chem Biol; Mitochondrial metabolism promotes adaptation to proteotoxic stress.; Authors - Peter Tsvetkov ; KO Validated
human	WB	J Biol Chem; Lipoylation is dependent on the ferredoxin FDX1 and dispensable under hypoxia in human cells; Authors - Pallavi R Joshi
human	WB	Front Oncol; ITGB1-mediated molecular landscape and cuproptosis phenotype induced the worse prognosis in diffuse gastric cancer; Authors - Xingyu Zhu
human	WB	Heliyon; Evaluation the role of cuproptosis-related genes in the pathogenesis, diagnosis and molecular subtypes identification of atherosclerosis; Authors - Mengxi Wang