FDXR Polyklonaler Antikörper

FDXR Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 15584-1-AP

Synonyme

Adrenodoxin reductase, ADXR, Aerredoxin Reductase, AR, FDXR, Ferredoxin NADP(+) reductase

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Galerie der Validierungsdaten

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  WB analysis using 15584-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15584-1-AP (FDXR antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of pig adrenal gland using 15584-1-AP

  pig adrenal gland tissue were subjected to SDS PAGE followed by western blot with 15584-1-AP (FDXR antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of human adrenal gland using 15584-1-AP

  human adrenal gland tissue were subjected to SDS PAGE followed by western blot with 15584-1-AP (FDXR antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 15584-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15584-1-AP (FDXR antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 15584-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15584-1-AP (FDXR antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushodengewebe, Hausschwein-Nebennierengewebe, humanes Nebennierengewebe, Rattenhodengewebe
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 2 publications below

Produktinformation

15584-1-AP bindet in WB, IHC, ELISA FDXR und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: FDXR fusion protein Ag7145
• Vollständiger Name: ferredoxin reductase
• Berechnetes Molekulargewicht: 54 kDa
• Beobachtetes Molekulargewicht: 48-58 kDa
• GenBank-Zugangsnummer: BC063493
• Gene symbol: FDXR
• Gene ID (NCBI): 2232
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Ferredoxin reductase (FDXR, also known as adrenodoxin reductase) is a mitochondrial membrane-associated flavoprotein. One of its functions is to transfer electrons from NADPH to the two human ferredoxin proteins, FDX1 and FDX2. FDXR is expressed in all tissues with the highest expression in tissues such as the adrenal cortex, that specialize in steroid hormone synthesis. Functionally, FDXR is suggested to be involved in various Functionally, FDXR is suggested to be involved in various. Notably, recent studies have shown that FDXR mutations are associated with mitochondrial disorders, probably due to its role in iron-sulfur cluster protein biosynthesis (PMID: 32304229). FDXR has 7 isoforms with the molecular weight of 48-58 kDa.

Publikationen

Species	Application	Title
human	WB	J Biol Chem; The neurosteroid pregnenolone is synthesized by a mitochondrial P450 enzyme other than CYP11A1 in human glial cells.; Authors - Yiqi Christina Lin
human	WB	Front Oncol; FDXR drives primary and endocrine-resistant tumor cell growth in ER+ breast cancer via CPT1A-mediated fatty acid oxidation; Authors - Chaojun Yan ; KD Validated