FEM1B Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

FEM1B Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(4)

Kat-Nr. : 19544-1-AP

Synonyme

FEM1-beta, FEM1 beta, fem 1 homolog b (C. elegans), F1A-alpha, F1AA

---

• 

  WB analysis of PC-3 using 19544-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 19544-1-AP (FEM1B antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse pancreas using 19544-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 19544-1-AP (FEM1B antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 19544-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 19544-1-AP (FEM1B antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of RAW264.7 using 19544-1-AP

  RAW264.7 cells were subjected to SDS PAGE followed by western blot with 19544-1-AP (FEM1B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human pancreas cancer using 19544-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 19544-1-AP (FEM1B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 19544-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 19544-1-AP (FEM1B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse pancreas using 19544-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 19544-1-AP (FEM1B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse pancreas using 19544-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 19544-1-AP (FEM1B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 19544-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 19544-1-AP (FEM1B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of PC-3 using 19544-1-AP

  Immunofluorescent analysis of (4% PFA) fixed PC-3 cells using FEM1B antibody (19544-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: PC-3-Zellen, Maus-Pankreasgewebe, Maushodengewebe
• Erfolgreiche Detektion in IHC: humanes Pankreaskarzinomgewebe, Maus-Pankreasgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: PC-3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IF: See 1 publications below

Produktinformation

19544-1-AP bindet in WB, IHC, IF/ICC, ELISA FEM1B und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: fem-1 homolog b (C. elegans)
• Berechnetes Molekulargewicht: 70 kDa
• Beobachtetes Molekulargewicht: 70 kDa
• GenBank-Zugangsnummer: NM_015322
• Gene symbol: FEM1B
• Gene ID (NCBI): 10116
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

FEM1B, also named F1AA and KIAA0396, belongs to the fem-1 family. FEM1B is a component of an E3 ubiquitin-protein ligase complex, in which it may act as a substrate recognition subunit. FEM1B is involved in apoptosis by acting as a death receptor-associated protein that mediates apoptosis. It is involved in glucose homeostasis in pancreatic islets.

Publikationen

Species	Application	Title
mouse	WB	Cell; A Cellular Mechanism to Detect and Alleviate Reductive Stress.; Authors - Andrew G Manford ; KD Validated
human	WB	J Am Chem Soc; Discovery of a Covalent FEM1B Recruiter for Targeted Protein Degradation Applications; Authors - Nathaniel J. Henning
	WB	Cell; Structural basis and regulation of the reductive stress response; Authors - Andrew G Manford
human	WB,IF	Mol Cell; Reactive oxygen species control protein degradation at the mitochondrial import gate; Authors - Rachael McMinimy