FEM1B Rekombinanter Antikörper

FEM1B Rekombinant Antikörper für IF/ICC, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

IF/ICC, FC (Intra), ELISA

Konjugation

Unkonjugiert

CloneNo.

240607D8

Kat-Nr. : 83810-1-RR

Synonyme

FEM1 beta, fem 1 homolog b (C. elegans), F1A-alpha, F1AA, F1A alpha

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  IF Staining of PC-3 using 83810-1-RR

  Immunofluorescent analysis of (4% PFA) fixed PC-3 cells using FEM1B antibody (83810-1-RR, Clone: 240607D8 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  IF Staining of PC-3 using 83810-1-RR

  Immunofluorescent analysis of (4% PFA) fixed PC-3 cells using FEM1B antibody (83810-1-RR, Clone: 240607D8 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  FC experiment of A431 using 83810-1-RR

  1x10^6 A431 cells were intracellularly stained with 0.25 ug FEM1B Recombinant antibody (83810-1-RR, Clone:240607D8) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF/ICC: PC-3-Zellen
• Erfolgreiche Detektion in FC (Intra): A431-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:125-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

83810-1-RR bindet in IF/ICC, FC (Intra), ELISA FEM1B und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: fem-1 homolog b (C. elegans)
• Berechnetes Molekulargewicht: 70 kDa
• GenBank-Zugangsnummer: NM_015322
• Gene symbol: FEM1B
• Gene ID (NCBI): 10116
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

FEM1B, also named F1AA and KIAA0396, belongs to the fem-1 family. FEM1B is a component of an E3 ubiquitin-protein ligase complex, in which it may act as a substrate recognition subunit. FEM1B is involved in apoptosis by acting as a death receptor-associated protein that mediates apoptosis. It is involved in glucose homeostasis in pancreatic islets.