FEM1C Rekombinanter Antikörper

FEM1C Rekombinant Antikörper für WB, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, FC (Intra), ELISA

Konjugation

Unkonjugiert

CloneNo.

240126G5

Kat-Nr. : 83536-1-RR

Synonyme

FEM1-gamma, FEM1A, FEM1 gamma, FEM1, FEM

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  WB analysis of HepG2 using 83536-1-RR

  HepG2 cells were subjected to SDS PAGE followed by western blot with 83536-1-RR (FEM1C antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  FC experiment of U2OS using 83536-1-RR

  1x10^6 U2OS cells were intracellularly stained with 0.25 ug Fem1c Recombinant Antibody (83536-1-RR, Clone:240126G5) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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  Affinity and Kinetic Characterization of 83536-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83536-1-RR against Human FEM1C were performed. The affinity constant is 0.896 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen
• Erfolgreiche Detektion in FC (Intra): U2OS-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

83536-1-RR bindet in WB, FC (Intra), ELISA FEM1C und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: FEM1C fusion protein Ag5099
• Vollständiger Name: fem-1 homolog c (C. elegans)
• Berechnetes Molekulargewicht: 69 kDa
• Beobachtetes Molekulargewicht: 69 kDa
• GenBank-Zugangsnummer: BC028369
• Gene symbol: FEM1C
• Gene ID (NCBI): 56929
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Protein fem-1 homolog C (FEM1C) is involved in protein modification and ubiquitination.Probable component of an E3 ubiquitin-protein ligase complex, in which it may act as a substrate recognition subunit.Highly expressed in kidney, cardiac tissue, skeletal muscle and testis. Expressed at lower levels in other tissues, including cartilage.This antibody specifically recognizes the 69kd FEM1C protein.