FEN1 Rekombinanter Antikörper

FEN1 Rekombinant Antikörper für WB, IHC, IF/ICC, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, FC (Intra), ELISA

Konjugation

Unkonjugiert

CloneNo.

242330C10

Kat-Nr. : 84916-5-RR

Synonyme

242330C10, EC:3.1.-.-, FEN 1, FEN-1, Flap structure-specific endonuclease 1

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  WB analysis using 84916-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84916-5-RR (FEN1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 84916-5-RR

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 84916-5-RR (FEN1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 84916-5-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using FEN1 antibody (84916-5-RR, Clone: 242330C10 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  IF Staining of HeLa using 84916-5-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using FEN1 antibody (84916-5-RR, Clone: 242330C10 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  FC experiment of HeLa using 84916-5-RR

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug FEN1 Recombinant antibody (84916-5-RR, Clone:242330C10) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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  Affinity and Kinetic Characterization of 84916-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84916-5-RR against Human FEN1 were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, HeLa-Zellen, Jurkat-Zellen, Maushodengewebe, NIH/3T3-Zellen, U-937-Zellen, Y79-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen
• Erfolgreiche Detektion in FC (Intra): HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:250-1:1000
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

84916-5-RR bindet in WB, IHC, IF/ICC, FC (Intra), ELISA FEN1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: FEN1 fusion protein Ag6552
• Vollständiger Name: flap structure-specific endonuclease 1
• Berechnetes Molekulargewicht: 43 kDa
• Beobachtetes Molekulargewicht: 48 kDa
• GenBank-Zugangsnummer: BC000323
• Gene symbol: FEN1
• Gene ID (NCBI): 2237
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

FEN1(Flap endonuclease-1) is the prototypical member of the 5'-nuclease superfamily, whose activities span a range of cellular pathways involved in DNA replication and genome maintenance (PMID: 22118811, 21496641, 20929870). FEN1 is a structure-selective metallonuclease essential for Okazaki fragment maturation through efficient removal of 5' flaps resulting from strand displacement during lagging-strand synthesis (PMID: 8144677, 9081985). FEN1 is overexpressed in multiple cancer types, and has been suggested both as a biomarker relating to prognosis and disease progression and as a potential therapeutic target (PMID: 19010819, 16879693, 19596913, 27526030).