FEN1 Rekombinanter Antikörper

FEN1 Rekombinant Antikörper für IF/ICC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

IF/ICC

Konjugation

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

242330C10

Kat-Nr. : CL647-84916-5

Synonyme

242330C10, EC:3.1.-.-, FEN 1, FEN-1, Flap structure-specific endonuclease 1

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  IF Staining of HeLa using CL647-84916-5

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 647 FEN1 antibody (CL647-84916-5, Clone: 242330C10 ) at dilution of 1:200.

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  IF Staining of NIH/3T3 using CL647-84916-5

  Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using CoraLite® Plus 647 FEN1 antibody (CL647-84916-5, Clone: 242330C10 ) at dilution of 1:200.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen, NIH/3T3-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL647-84916-5 bindet in IF/ICC FEN1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: FEN1 fusion protein Ag6552
• Vollständiger Name: flap structure-specific endonuclease 1
• Berechnetes Molekulargewicht: 43 kDa
• GenBank-Zugangsnummer: BC000323
• Gene symbol: FEN1
• Gene ID (NCBI): 2237
• Konjugation: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 654 nm / 674 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

FEN1(Flap endonuclease-1) is the prototypical member of the 5'-nuclease superfamily, whose activities span a range of cellular pathways involved in DNA replication and genome maintenance (PMID: 22118811, 21496641, 20929870). FEN1 is a structure-selective metallonuclease essential for Okazaki fragment maturation through efficient removal of 5' flaps resulting from strand displacement during lagging-strand synthesis (PMID: 8144677, 9081985). FEN1 is overexpressed in multiple cancer types, and has been suggested both as a biomarker relating to prognosis and disease progression and as a potential therapeutic target (PMID: 19010819, 16879693, 19596913, 27526030).