Kindlin 3 Polyklonaler Antikörper

Kindlin 3 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

1

Kat-Nr. : 18131-1-AP

Synonyme

Fermitin family homolog 3, FERMT3, KIND3, Kindlin 3, MIG 2, MIG2 like protein, MIG2B, Unc 112 related protein 2, UNC112C, URP2, URP2SF

---

Galerie der Validierungsdaten

• 

  WB analysis of Jurkat using 18131-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 18131-1-AP (FERMT3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 18131-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 18131-1-AP (FERMT3 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 18131-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 18131-1-AP (FERMT3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 18131-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 18131-1-AP (FERMT3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 18131-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 18131-1-AP (FERMT3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 18131-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 18131-1-AP (FERMT3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human lymphoma using 18131-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 18131-1-AP (FERMT3 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lymphoma using 18131-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 18131-1-AP (FERMT3 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 18131-1-AP

  Immunofluorescent analysis of HeLa cells using 18131-1-AP (FERMT3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, Raji-Zellen
• Erfolgreiche Detektion in IHC: humanes Lymphomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IF: See 1 publications below

Produktinformation

18131-1-AP bindet in WB, IHC, IF, ELISA Kindlin 3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Kindlin 3 fusion protein Ag12713
• Vollständiger Name: fermitin family homolog 3 (Drosophila)
• Berechnetes Molekulargewicht: 76 kDa
• Beobachtetes Molekulargewicht: 72-76 kDa
• GenBank-Zugangsnummer: BC004347
• Gene symbol: FERMT3
• Gene ID (NCBI): 83706
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Kindlin-3 (FERMT-3) is known to be central in hemostasis and thrombosis control and its deficiency disrupts platelet aggregation and causes Leukocyte Adhesion Deficiency disease. Point mutations in the kindlin-3 gene have been identified in humans with a rare inherited Integrin Activation efficiency Disease (IADD), also designated as Leukocyte Adhesion Deficiency syndrome (LAD-III or LADI variant). Kindlin-3 expression is restricted to cells of hematopoietic origin.

Publikationen

Species	Application	Title
	IF	PLoS Biol; Non-muscle myosins control radial glial basal endfeet to mediate interneuron organization; Authors - Brooke R D'Arcy