FES Polyklonaler Antikörper

FES Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 28194-1-AP

Synonyme

feline sarcoma oncogene, FES, FPS, Proto oncogene c Fes, Proto oncogene c Fps

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  WB analysis using 28194-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28194-1-AP (FES antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 28194-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 28194-1-AP (FES antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 28194-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 28194-1-AP (FES antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 28194-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 28194-1-AP (FES antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 28194-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 28194-1-AP (FES antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HL-60-Zellen, THP-1-Zellen, U-937-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

28194-1-AP bindet in WB, IHC, ELISA FES und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: FES fusion protein Ag28095
• Vollständiger Name: feline sarcoma oncogene
• Berechnetes Molekulargewicht: 822 aa, 94 kDa
• Beobachtetes Molekulargewicht: 93 kDa, 79 kDa, 87 kDa, 85 kDa
• GenBank-Zugangsnummer: BC035357
• Gene symbol: FES
• Gene ID (NCBI): 2242
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Feline sarcoma oncogene protein (FES) is a member of the FES/FES-related (FER) subfamily of non-receptor protein tyrosine kinases. FES was originally identified a retroviral oncogene in avian and feline retroviruses. Articles reported that FES plays a role in growth and differentiation of myeloid hematopoietic cells, vascular endothelial cells and neurons. And it also involved in the regulation of cytoskeletal rearrangement (PMID:15929003). Addition, a recent study suggests that FES may function both tumorigenic and tumor-suppressive effects (PMID: 28952025).