FEZ2 Rekombinanter Antikörper

FEZ2 Rekombinant Antikörper für WB, IHC, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, FC (Intra), ELISA

Konjugation

Unkonjugiert

CloneNo.

240248B10

Kat-Nr. : 83407-7-PBS

Synonyme

240248B10, Fasciculation and elongation protein zeta-2, HUM3CL, Zygin 2, Zygin II

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  WB analysis using 83407-7-RR (same clone as 83407-7-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83407-7-RR (FEZ2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83407-7-PBS in a different storage buffer formulation.

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  IHC staining of human ovary cancer using 83407-7-RR (same clone as 83407-7-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83407-7-RR (FEZ2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83407-7-PBS in a different storage buffer formulation.

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  FC experiment of A431 using 83407-7-RR (same clone as 83407-7-PBS)

  1x10^6 A431 cells were intracellularly stained with 0.25 ug Fez2 Recombinant Antibody (83407-7-RR, Clone:240248B10) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83407-7-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83407-7-PBS

  Biolayer interferometry (BLl) kinetic assay of 83407-7-PBS against Human FEZ2 was performed. The affinity constant is 0.212 nM.

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  Affinity and Kinetic Characterization of 83407-7-RR

  Biolayer interferometry (BLl) kinetic assays of 83407-7-RR against Human FEZ2 were performed. The affinity constant is below 1 nM.

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Geprüfte Anwendungen

Produktinformation

83407-7-PBS bindet in WB, IHC, FC (Intra), ELISA FEZ2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: FEZ2 fusion protein Ag15370
• Vollständiger Name: fasciculation and elongation protein zeta 2 (zygin II)
• Berechnetes Molekulargewicht: 353 aa, 40 kDa
• Beobachtetes Molekulargewicht: ~50 kDa
• GenBank-Zugangsnummer: BC110386
• Gene symbol: FEZ2
• Gene ID (NCBI): 9637
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

FEZ2 is an ortholog of the C. elegans unc-76 gene, which is necessary for normal axonal bundling and elongation within axon bundles.