- Featured Product
- KD/KO Validated
FFAR2 Polyklonaler Antikörper
FFAR2 Polyklonal Antikörper für WB, IHC, ELISA
Wirt / Isotyp
Kaninchen / IgG
Getestete Reaktivität
Hausschwein, human und mehr (2)
Anwendung
WB, IHC, IF, IP, ELISA
Konjugation
Unkonjugiert
Kat-Nr. : 19952-1-AP
Synonyme
Geprüfte Anwendungen
| Erfolgreiche Detektion in WB | THP-1-Zellen, HCT 116-Zellen, HeLa-Zellen, Hausschwein-Lebergewebe | 
| Erfolgreiche Detektion in IHC | humanes Milzgewebe,  humanes Kolongewebe Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen. | 
Empfohlene Verdünnung
| Anwendung | Verdünnung | 
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 | 
| Immunhistochemie (IHC) | IHC : 1:50-1:500 | 
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Veröffentlichte Anwendungen
| KD/KO | See 1 publications below | 
| WB | See 28 publications below | 
| IHC | See 2 publications below | 
| IF | See 4 publications below | 
| IP | See 1 publications below | 
Produktinformation
19952-1-AP bindet in WB, IHC, IF, IP, ELISA FFAR2 und zeigt Reaktivität mit Hausschwein, human
| Getestete Reaktivität | Hausschwein, human | 
| In Publikationen genannte Reaktivität | human, Hausschwein, Maus, Ratte | 
| Wirt / Isotyp | Kaninchen / IgG | 
| Klonalität | Polyklonal | 
| Typ | Antikörper | 
| Immunogen | Peptid | 
| Vollständiger Name | free fatty acid receptor 2 | 
| Berechnetes Molekulargewicht | 37 kDa | 
| Beobachtetes Molekulargewicht | 50 kDa, 37 kDa | 
| GenBank-Zugangsnummer | NM_005306 | 
| Gene symbol | FFAR2 | 
| Gene ID (NCBI) | 2867 | 
| Konjugation | Unkonjugiert | 
| Form | Liquid | 
| Reinigungsmethode | Antigen-Affinitätsreinigung | 
| Lagerungspuffer | PBS with 0.02% sodium azide and 50% glycerol | 
| Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. | 
Hintergrundinformationen
Free fatty acid receptors (FFAR) play significant roles in various physiological processes through interaction with their ligands, fatty acids. Free fatty acid receptor 2 (FFAR2, also known as FFA2 or GPR43) is a receptor for short-chain fatty acids (SCFAs) and plays a role in the regulation of whole-body energy homeostasis and intestinal immunity (PMID: 12684041). It has been considered a therapeutic target for metabolic and inflammatory conditions (PMID: 23589301). FFAR2 has a calculated molecular weight of 37 kDa and can be glycosylated. The higher apparent molecular weight of 50 kDa has been reported, probably due to glycosylation (PMID: 31707282; 28131568).
Protokolle
| PRODUKTSPEZIFISCHE PROTOKOLLE | |
|---|---|
| WB protocol for FFAR2 antibody 19952-1-AP | Protokoll herunterladen | 
| IHC protocol for FFAR2 antibody 19952-1-AP | Protokoll herunterladenl | 
| STANDARD-PROTOKOLLE | |
|---|---|
| Klicken Sie hier, um unsere Standardprotokolle anzuzeigen | 
Publikationen
| Species | Application | Title | 
|---|---|---|
| Food Chem X Dendrobium officinale leaf polysaccharides ameliorated hyperglycemia and promoted gut bacterial associated SCFAs to alleviate type 2 diabetes in adult mice. | ||
| Chin Med The relationship between gut microbiota and susceptibility to type 2 diabetes mellitus in rats | ||
| Chin Med Shaoyao Decoction reduced T lymphocyte activation by regulating of intestinal flora and 5-hydroxytryptamine metabolism in ulcerative colitis | ||
| Pharmaceuticals (Basel) Xiao Cheng Qi Decoction, an Ancient Chinese Herbal Mixture, Relieves Loperamide-Induced Slow-Transit Constipation in Mice: An Action Mediated by Gut Microbiota | ||
| Phytomedicine The beneficial effects of Polygonatum sibiricum Red. superfine powder on metabolic hypertensive rats via gut-derived LPS/TLR4 pathway inhibition | ||
| Food Funct EPA and DHA differentially coordinate the crosstalk between host and gut microbiota and block DSS-induced colitis in mice by a reinforced colonic mucus barrier. | 
Rezensionen
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
| FH Christin (Verified Customer) (01-26-2025) | Antibody used to study G protein coupled receptors responsible for uptake of short chain fatty acids in human iPSC-derived microglia and macrophages. 
 | 






