FFAR2 Polyklonaler Antikörper

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• KD/KO Validated

FFAR2 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Hausschwein, human und mehr (2)

Anwendung

WB, IHC, IF, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(33)

Rezensionen (1)

Kat-Nr. : 19952-1-AP

Synonyme

FFA2, FFA2R, free fatty acid receptor 2, G protein coupled receptor 43, GPCR43

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  WB analysis using 19952-1-AP

  THP-1 cells were subjected to SDS PAGE followed by western blot with 19952-1-AP (FFAR2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 19952-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19952-1-AP (FFAR2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of pig liver using 19952-1-AP

  pig liver tissue were subjected to SDS PAGE followed by western blot with 19952-1-AP (FFAR2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human spleen using 19952-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 19952-1-AP (FFAR2 Antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human spleen using 19952-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 19952-1-AP (FFAR2 Antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human colon using 19952-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 19952-1-AP (FFAR2 Antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human colon using 19952-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 19952-1-AP (FFAR2 Antibody) at dilution of 1:100 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: THP-1-Zellen, HCT 116-Zellen, HeLa-Zellen, Hausschwein-Lebergewebe
• Erfolgreiche Detektion in IHC: humanes Milzgewebe, humanes Kolongewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 28 publications below
• IHC: See 2 publications below
• IF: See 4 publications below
• IP: See 1 publications below

Produktinformation

19952-1-AP bindet in WB, IHC, IF, IP, ELISA FFAR2 und zeigt Reaktivität mit Hausschwein, human

• Getestete Reaktivität: Hausschwein, human
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: free fatty acid receptor 2
• Berechnetes Molekulargewicht: 37 kDa
• Beobachtetes Molekulargewicht: 50 kDa, 37 kDa
• GenBank-Zugangsnummer: NM_005306
• Gene symbol: FFAR2
• Gene ID (NCBI): 2867
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Free fatty acid receptors (FFAR) play significant roles in various physiological processes through interaction with their ligands, fatty acids. Free fatty acid receptor 2 (FFAR2, also known as FFA2 or GPR43) is a receptor for short-chain fatty acids (SCFAs) and plays a role in the regulation of whole-body energy homeostasis and intestinal immunity (PMID: 12684041). It has been considered a therapeutic target for metabolic and inflammatory conditions (PMID: 23589301). FFAR2 has a calculated molecular weight of 37 kDa and can be glycosylated. The higher apparent molecular weight of 50 kDa has been reported, probably due to glycosylation (PMID: 31707282; 28131568).

Publikationen

Species	Application	Title
mouse	WB	Food Chem X; Dendrobium officinale leaf polysaccharides ameliorated hyperglycemia and promoted gut bacterial associated SCFAs to alleviate type 2 diabetes in adult mice.; Authors - Jingyu Fang
rat	WB	Chin Med; The relationship between gut microbiota and susceptibility to type 2 diabetes mellitus in rats; Authors - Yongcheng An
human	WB	Chin Med; Shaoyao Decoction reduced T lymphocyte activation by regulating of intestinal flora and 5-hydroxytryptamine metabolism in ulcerative colitis; Authors - Jianhua Zhen
mouse	WB,IHC	Pharmaceuticals (Basel); Xiao Cheng Qi Decoction, an Ancient Chinese Herbal Mixture, Relieves Loperamide-Induced Slow-Transit Constipation in Mice: An Action Mediated by Gut Microbiota; Authors - Amanguli Tuohongerbieke
rat	WB	Phytomedicine; The beneficial effects of Polygonatum sibiricum Red. superfine powder on metabolic hypertensive rats via gut-derived LPS/TLR4 pathway inhibition; Authors - Jie Su
mouse	WB	Food Funct; EPA and DHA differentially coordinate the crosstalk between host and gut microbiota and block DSS-induced colitis in mice by a reinforced colonic mucus barrier.; Authors - Jian Fang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Christin (Verified Customer) (01-26-2025)

Antibody used to study G protein coupled receptors responsible for uptake of short chain fatty acids in human iPSC-derived microglia and macrophages.

• Applications: Western Blot
• Primary Antibody Dilution: 1:750 in milk
• Cell Tissue Type: Human iPSC-derived macrophages and microglia