Fibrinogen Beta Chain Polyklonaler Antikörper

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• KD/KO Validated

Fibrinogen Beta Chain Polyklonal Antikörper für IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

10

Kat-Nr. : 16747-1-AP

Synonyme

FGB, fibrinogen beta chain

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Galerie der Validierungsdaten

• 

  WB analysis of mouse heart using 16747-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 16747-1-AP (Fibrinogen beta chain antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 16747-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 16747-1-AP (Fibrinogen beta chain antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 16747-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 16747-1-AP (Fibrinogen beta chain antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 16747-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 16747-1-AP (Fibrinogen beta chain antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human kidney using 16747-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16747-1-AP (Fibrinogen beta chain antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 16747-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16747-1-AP (Fibrinogen beta chain antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausherzgewebe, Maushirngewebe, Mauslebergewebe, Mausnierengewebe
• Erfolgreiche Detektion in IHC: humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 8 publications below
• IHC: See 3 publications below
• IF: See 4 publications below

Produktinformation

16747-1-AP bindet in WB, IHC, IF, ELISA Fibrinogen Beta Chain und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte, Plasmodium falciparum
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Fibrinogen Beta Chain fusion protein Ag10169
• Vollständiger Name: fibrinogen beta chain
• Berechnetes Molekulargewicht: 491 aa, 56 kDa
• Beobachtetes Molekulargewicht: 50 kDa
• GenBank-Zugangsnummer: BC107766
• Gene symbol: FGB
• Gene ID (NCBI): 2244
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Fibrinogen is a soluble plasma glycoprotein synthesized in the liver. It is composed of two sets of three structurally different subunits: alpha (FGA), beta (FGB), gamma (FGG). Fibrinogen is converted by thrombin into fibrin during blood coagulation. Fibrinogen and fibrin play overlapping roles in blood clotting, fibrinolysis, cellular and matrix interactions, the inflammatory response, wound healing, and neoplasia (PMID: 16102057). FGB is the beta chain of fibrinogen. During conversion of fibrinogen to fibrin, fibrinopeptide B is cleaved from FGB by thrombin. Mutations in the gene of FGB lead to several disorders, including afibrinogenemia, dysfibrinogenemia, hypodysfibrinogenemia and thrombotic tendency.

Publikationen

Species	Application	Title
human,rat	IHC,IF	Blood; Fibrinogen β-derived Bβ(15-42) peptide protects against kidney ischemia/reperfusion injury.; Authors - Krishnamoorthy Aparna A
human	WB,IHC,IF	Front Neurosci; Fibrinogen Chains Intrinsic to the Brain.; Authors - Eugene V Golanov ; KD Validated
	WB,IF	Reproduction; Formation of fibrin at sights of conceptus adhesion in the ewe.; Authors - Yuta Matsuno
	WB	Biochem Biophys Res Commun; Characterization of lncRNA functioning in ovine conceptuses and endometria during the peri-implantation period.; Authors - Yuta Matsuno
Plasmodium falciparum	WB	Malar J; The malaria parasite Plasmodium falciparum in red blood cells selectively takes up serum proteins that affect host pathogenicity.; Authors - Takahiro Tougan
mouse,human	IF	Brain Res; Accelerated accumulation of fibrinogen peptide chains with Aβ deposition in Alzheimer's disease (AD) mice and human AD brains.; Authors - Zhihong Bian

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