FGD1 Rekombinanter Antikörper

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• KD/KO Validated

FGD1 Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

242314C3

Kat-Nr. : 84783-2-RR

Synonyme

242314C3, FGDY, FYVE, RhoGEF and PH domain-containing protein 1, Rho/Rac GEF, ZFYVE3

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  WB analysis using 84783-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84783-2-RR (FGD1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of U2OS using 84783-2-RR

  WB result of FGD1 antibody (84783-2-RR; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FGD1 transfected U2OS cells.

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  Affinity and Kinetic Characterization of 84783-2-RR

  Biolayer interferometry (BLl) kinetic assays of 84783-2-RR against Human FGD1 were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: SK-N-SH cells, BxPC-3-Zellen, U2OS-Zellen, U-87 MG-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

84783-2-RR bindet in WB, ELISA FGD1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: FGD1 fusion protein Ag3861
• Vollständiger Name: FYVE, RhoGEF and PH domain containing 1
• Berechnetes Molekulargewicht: 901 aa, 91 kDa
• Beobachtetes Molekulargewicht: 130 kDa
• GenBank-Zugangsnummer: BC034530
• Gene symbol: FGD1
• Gene ID (NCBI): 2245
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

FGD1 is also named as FGDY (Faciogenital dysplasia 1 protein), ZFYVE3 (Zinc finger FYVE domain-containing protein 3), Rho/Rac guanine nucleotide exchange factor FGD1 (Rho/Rac GEF), FYVE, RhoGEF and PH domain-containing protein 1. FGD1 activates CDC42, a member of the Ras-like family of Rho- and Rac proteins, by exchanging bound GDP for free GTP. And FGD1 plays a role in regulating the actin cytoskeleton and cell shape. (PMID: 8969170). FGD1 was markedly overexpressed and might be a prognostic biomarker in osteosarcoma patient specimens (PMID: 32194840). FGD1 is specific for the Rho GTPase cell division cycle 42 (CDC42) (PMID: 22854039).