FGF12 Polyklonaler Antikörper

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• KD/KO Validated

FGF12 Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 13784-1-AP

Synonyme

FGF 12, FGF12, FGF12B, FHF 1, FHF1, Myocyte activating factor

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Galerie der Validierungsdaten

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  WB analysis using 13784-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13784-1-AP (FGF12 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 13784-1-AP

  IP result of anti-FGF12(IP:13784-1-AP, 4ug; Detection:13784-1-AP 1:6000) with mouse brain tissue lysate 1280 ug.

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  IHC staining of mouse brain using 13784-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13784-1-AP (FGF12 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of mouse brain using 13784-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13784-1-AP (FGF12 Antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, Rattenhirngewebe
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:12000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IF: See 2 publications below

Produktinformation

13784-1-AP bindet in WB, IP, IHC, IF, ELISA FGF12 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: FGF12 fusion protein Ag4797
• Vollständiger Name: fibroblast growth factor 12
• Berechnetes Molekulargewicht: 181 aa, 20 kDa
• Beobachtetes Molekulargewicht: 20-22 kDa
• GenBank-Zugangsnummer: BC022524
• Gene symbol: FGF12
• Gene ID (NCBI): 2257
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

FGF12 is a member of the FGF family. FGF family members play important roles in embryogenesis, angiogenesis, and wound repair. FGF12 lacks the N-terminal signal sequence present in most of the FGF family members, but it contains clusters of basic residues that have been demonstrated to act as a nuclear localization signal. When transfected into mammalian cells, this protein accumulated in the nucleus, but was not secreted. FGF12 plays an intracellular role in the inhibition of radiation-induced apoptosis. FGF12 is expressed abundantly in developing and adult nervous systems; therefore, FGF12 was thought to be related to nervous system development and function.

Publikationen

Species	Application	Title
rat	IF	Neurobiol Dis; Decreased expression of synaptic genes in the vestibular ganglion of rodents following subchronic ototoxic stress; Authors - Erin A Greguske
human	WB	Epilepsia; Early onset epilepsy and sudden unexpected death in epilepsy with cardiac arrhythmia in mice carrying the early infantile epileptic encephalopathy 47 gain-of-function FHF1(FGF12) missense mutation.; Authors - Jana Velíšková
mouse	WB,IF	Sci Rep; Fibroblast growth factor 12 is expressed in spiral and vestibular ganglia and necessary for auditory and equilibrium function.; Authors - Yukiko Hanada ; KO Validated