FGF18 Polyklonaler Antikörper

FGF18 Polyklonal Antikörper für IF, IHC,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 11495-1-AP

Synonyme

FGF 18, FGF18, fibroblast growth factor 18, ZFGF5

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Galerie der Validierungsdaten

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  IHC staining of mouse kidney using 11495-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 11495-1-AP (FGF18 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 11495-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 11495-1-AP (FGF18 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse heart using 11495-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 11495-1-AP (FGF18 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 11495-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 11495-1-AP (FGF18 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse embryo using 11495-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 11495-1-AP (FGF18 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse embryo using 11495-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 11495-1-AP (FGF18 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse embryo using 11495-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 11495-1-AP (FGF18 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse embryo using 11495-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 11495-1-AP (FGF18 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 11495-1-AP

  Immunofluorescent analysis of HepG2 cells using 11495-1-AP (FGF18 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: Mausnierengewebe, Mausembryogewebe, Mausherzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Produktinformation

11495-1-AP bindet in WB, IHC, IF, ELISA FGF18 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: FGF18 fusion protein Ag2041
• Vollständiger Name: fibroblast growth factor 18
• Berechnetes Molekulargewicht: 207 aa, 24 kDa
• GenBank-Zugangsnummer: BC006245
• Gene symbol: FGF18
• Gene ID (NCBI): 8817
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

FGF18 is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth, and invasion. It has been shown in vitro that this protein is able to induce neurite outgrowth in PC12 cells. Studies of the similar proteins in mouse and chick suggested that this protein is a pleiotropic growth factor that stimulates proliferation in a number of tissues, most notably the liver and small intestine. Knockout studies of the similar gene in mice implied the role of this protein in regulating proliferation and differentiation of midline cerebellar structures.

Publikationen

Species	Application	Title
mouse	WB, IHC	Front Pharmacol; Suppression of FGF5 and FGF18 Expression by Cholesterol-Modified siRNAs Promotes Hair Growth in Mice.; Authors - Jungang Zhao
human	IHC	J Exp Clin Cancer Res; HDAC7 promotes NSCLC proliferation and metastasis via stabilization by deubiquitinase USP10 and activation of β-catenin-FGF18 pathway.; Authors - Kai Guo
mouse	WB,IF	Ann Transl Med; Cucurbitacin promotes hair growth in mice by inhibiting the expression of fibroblast growth factor 18; Authors - Keke Guo