FGF19 Rekombinanter Antikörper

FGF19 Rekombinant Antikörper für WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Konjugation

Unkonjugiert

CloneNo.

241740E4

Kat-Nr. : 84536-3-PBS

Synonyme

UNQ334/PRO533, Fibroblast growth factor 19, FGF-19, FGF 19, 241740E4

---

• 

  WB analysis of HT-29 using 84536-3-RR (same clone as 84536-3-PBS)

  HT-29 cells were subjected to SDS PAGE followed by western blot with 84536-3-RR (FGF19 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84536-3-PBS in a different storage buffer formulation.

• 

  Sandwich ELISA standard curve of MP01398-1

  Sandwich ELISA standard curve of MP01398-1, Human FGF19 Recombinant Matched Antibody Pair - PBS only. 84536-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard HZ-1330. 84536-2-PBS was HRP conjugated as the detection antibody. Range: 31.3-1000 pg/mL.

• 

  Sample test of MP01398-1

  Serum of six individual healthy human donors was measured. The FGF19 concentration of detected samples was determined to be 199.4 pg/mL with a range of 136.6-250.1 pg/mL.

• 

  Sample test of MP01398-1

  HT-29 cells were cultured in DMEM supplemented with 10% fetal bovine serum, 2.5 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. An aliquot of the cell culture supernate was removed, assayed for human FGF19, and measured 32.7 ng/mL.

• 

  Cytometric bead array standard curve of MP01398-1

  Cytometric bead array standard curve of MP01398-1, FGF19 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84536-3-PBS. Detection antibody: 84536-2-PBS. Standard: HZ-1330. Range: 0.078-20 ng/mL.

• 

  Affinity and Kinetic Characterization of 84536-3-RR

  Biolayer interferometry (BLl) kinetic assays of 84536-3-RR against Human FGF19 were performed. The affinity constant is 0.323 nM.

---

Geprüfte Anwendungen

Produktinformation

84536-3-PBS bindet in WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test FGF19 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: FGF19 fusion protein HZ-1330
• Vollständiger Name: fibroblast growth factor 19
• Berechnetes Molekulargewicht: 216 aa, 24 kDa
• Beobachtetes Molekulargewicht: 24 kDa
• GenBank-Zugangsnummer: BC017664
• Gene symbol: FGF19
• Gene ID (NCBI): 9965
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Fibroblast growth factor 19 (FGF19) is a member of the fibroblast growth factor family, which consists of proteins with diverse effects on development, organogenesis, and metabolism. FGF19 is unique within this family as it functions as an endocrine protein, circulating in the blood and acting on distant target tissues. It is produced in the distal ileum and secreted into the circulation after a meal, playing a role in controlling the enterohepatic circulation of bile acids and regulating hepatic protein and glycogen metabolism in an insulin-independent manner. .