FGF21 Polyklonaler Antikörper

FGF21 Polyklonal Antikörper für IF, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 26272-1-AP

Synonyme

FGF 21, FGF21, fibroblast growth factor 21

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Galerie der Validierungsdaten

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  IHC staining of human lung cancer using 26272-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 26272-1-AP (FGF21 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 26272-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 26272-1-AP (FGF21 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 26272-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 26272-1-AP (FGF21 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human colon using 26272-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 26272-1-AP (FGF21 Antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of mouse liver using 26272-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using FGF21 antibody (26272-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of mouse liver using 26272-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using FGF21 antibody (26272-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe, humanes Kolongewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: Mauslebergewebe

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IF: See 1 publications below

Produktinformation

26272-1-AP bindet in IHC, IF, ELISA FGF21 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: FGF21 fusion protein Ag24064
• Vollständiger Name: fibroblast growth factor 21
• Berechnetes Molekulargewicht: 22 kDa
• GenBank-Zugangsnummer: BC018404
• Gene symbol: FGF21
• Gene ID (NCBI): 26291
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Fibroblast growth factor 21 (FGF21), a member of the FGF superfamily, has emerged as an important metabolic regulator. FGF21 is produced in the liver, adipocytes and skeletal muscle and regulates glucose, energy and lipid metabolism. FGF21 is a stress-responsive hepatokine which is induced in the liver as a response to injury, regeneration, hepatocarcinogenesis and during kidney dysfunction. FGF21 is primarily metabolized in the kidney and renal secretion is the major route of FGF21 elimination. Its plasma levels increase according to the decline in renal function in patients with chronic and acute renal dysfunction.

Publikationen

Species	Application	Title
mouse		Nat Commun; The mitochondrial fusion protein OPA1 is dispensable in the liver and its absence induces mitohormesis to protect liver from drug-induced injury; Authors - Hakjoo Lee
human		Biomed Pharmacother; Dihydroartemisinin inhibits ER stress-mediated mitochondrial pathway to attenuate hepatocyte lipoapoptosis via blocking the activation of the PI3K/Akt pathway.; Authors - Xingran Chen
rat	IF	Cell Death Dis; FGF21 augments autophagy in random-pattern skin flaps via AMPK signaling pathways and improves tissue survival.; Authors - Kailiang Zhou