FGF9 Polyklonaler Antikörper

FGF9 Polyklonal Antikörper für IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 26554-1-AP

Synonyme

FGF-9, FGF 9, Heparin-binding growth factor 9, HBGF-9, Glia-activating factor

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  IHC staining of human stomach using 26554-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 26554-1-AP (FGF9 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human stomach using 26554-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 26554-1-AP (FGF9 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human small intestine using 26554-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 26554-1-AP (FGF9 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human small intestine using 26554-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 26554-1-AP (FGF9 antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human colon cancer using 26554-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 26554-1-AP (FGF9 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 26554-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 26554-1-AP (FGF9 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 26554-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 26554-1-AP (FGF9 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 26554-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 26554-1-AP (FGF9 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of MCF-7 using 26554-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using FGF9 antibody (26554-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: humanes Magengewebe, humanes Kolonkarzinomgewebe, humanes Prostatakarzinomgewebe, humanes Dünndarmgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: MCF-7-Zellen

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

26554-1-AP bindet in IHC, IF/ICC, ELISA FGF9 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: FGF9 fusion protein Ag24244
• Vollständiger Name: fibroblast growth factor 9 (glia-activating factor)
• Berechnetes Molekulargewicht: 208 aa, 23 kDa
• GenBank-Zugangsnummer: BC069692
• Gene symbol: FGF9
• Gene ID (NCBI): 2254
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Fibroblast growth factor 9 (FGF9) is a critically important and elegantly regulated growth factor that was first identified during a screen for factors acting on cells of the central nervous system (CNS). Over the subsequent two decades of research, FGF9 has been revealed to play a significant role in various biological processes. A key feature of its control is reciprocal compartmentalization, particularly during development, with the epithelium serving as a dominant source and mesenchyme as a prime target. This mesenchyme selectivity is achieved through the high affinity of FGF9 to the IIIc isoforms of FGFR1, 2, and 3. FGF9 is widely expressed in the embryo, including in the developing heart and lungs, and more selectively in adult tissues, such as the CNS and kidneys.