FGFR1 Polyklonaler Antikörper

FGFR1 Polyklonal Antikörper für IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 30358-1-AP

Synonyme

bFGF R 1, BFGFR, bFGF-R-1, CD331, CEK

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  IP experiment of SH-SY5Y using 30358-1-AP

  IP result of anti-FGFR1 (IP:30358-1-AP, 4ug; Detection:30358-1-AP 1:300) with SH-SY5Y cells lysate 770 ug.

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  IHC staining of human colon using 30358-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 30358-1-AP (FGFR1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 30358-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 30358-1-AP (FGFR1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 30358-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 30358-1-AP (FGFR1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 30358-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 30358-1-AP (FGFR1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat kidney using 30358-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 30358-1-AP (FGFR1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat kidney using 30358-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 30358-1-AP (FGFR1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of SH-SY5Y using 30358-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using FGFR1 antibody (30358-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche IP: SH-SY5Y-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolongewebe, Maushirngewebe, Rattennierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: SH-SY5Y-Zellen

Empfohlene Verdünnung

• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

30358-1-AP bindet in IHC, IF/ICC, IP, ELISA FGFR1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: fibroblast growth factor receptor 1
• Berechnetes Molekulargewicht: 820 aa, 91 kDa
• Beobachtetes Molekulargewicht: 80 kDa, 95 kDa, 105 kDa
• GenBank-Zugangsnummer: BC018128
• Gene symbol: FGFR1
• Gene ID (NCBI): 2260
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Fibroblast growth factor receptor 1 (FGFR1) is a tyrosine-protein kinase that acts as a cell-surface receptor for fibroblast growth factors. FGFR1 has been implicated in diverse biological processes including embryonic development, cell proliferation, differentiation, migration, and tumorigenesis. Mutations in the FGFR1 gene have been associated with Pfeiffer syndrome, Jackson-Weiss syndrome, Antley-Bixler syndrome, osteoglophonic dysplasia, squamous cell lung cancer, and autosomal dominant Kallmann syndrome. Alternatively spliced variants that encode different protein isoforms have been described.