FGFR1OP Monoklonaler Antikörper

FGFR1OP Monoklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

1F1D8

Kat-Nr. : 67207-1-Ig

Synonyme

Centrosomal protein 43, CEP43, FGFR1 oncogene partner, FOP

---

• 

  WB analysis using 67207-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67207-1-Ig (FGFR1OP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 67207-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67207-1-Ig (FGFR1OP antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of BT-549 using 67207-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed BT-549 cells using FGFR1OP antibody (67207-1-Ig, Clone: 1F1D8 ) at dilution of 1:2400 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, K-562-Zellen, U2OS-Zellen
• Erfolgreiche Detektion in IF/ICC: BT-549 cells

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:1200-1:4800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

67207-1-Ig bindet in WB, IF/ICC, ELISA FGFR1OP und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: FGFR1OP fusion protein Ag28671
• Vollständiger Name: FGFR1 oncogene partner
• Berechnetes Molekulargewicht: 43 kDa
• Beobachtetes Molekulargewicht: 52 kDa
• GenBank-Zugangsnummer: BC011902
• Gene symbol: FGFR1OP
• Gene ID (NCBI): 11116
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

FGFR1OP, also named as FOP, is a centrosomal protein that is involved in the anchoring of microtubules to subcellular structures. Three isoforms have been produced by alternative splicing. A chromosomal aberration involving FGFR1OP may be a cause of stem cell myeloproliferative disorder (MPD). MPD is characterized by myeloid hyperplasia, eosinophilia and T-cell or B-cell lymphoblastic lymphoma and progresses to acute myeloid leukemia.