FGFR2/CD332 Polyklonaler Antikörper

FGFR2/CD332 Polyklonal Antikörper für IHC, IF/ICC, FC (Intra), Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

IHC, IF/ICC, FC (Intra), Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 13042-1-PBS

Synonyme

FGFR2, BEK, BFR 1, CD332, CEK3

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  IHC staining of human breast cancer using 13042-1-AP (same clone as 13042-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13042-1-AP (FGFR2 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 13042-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 13042-1-AP (same clone as 13042-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13042-1-AP (FGFR2 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 13042-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 13042-1-AP (same clone as 13042-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 13042-1-AP (FGFR2 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 13042-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 13042-1-AP (same clone as 13042-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 13042-1-AP (FGFR2 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 13042-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 13042-1-AP (same clone as 13042-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using FGFR2 antibody (13042-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 13042-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 13042-1-AP (same clone as 13042-1-PBS)

  Immunofluorescent analysis of HepG2 cells using 13042-1-AP (FGFR2 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 13042-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 13042-1-AP (same clone as 13042-1-PBS)

  Immunofluorescent analysis of HepG2 cells, using FGFR2 antibody 13042-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye). This data was developed using the same antibody clone with 13042-1-PBS in a different storage buffer formulation.

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  FC experiment of HepG2 using 13042-1-AP (same clone as 13042-1-PBS)

  1X10^6 HepG2 cells were intracellularly stained with 0.8 ug Anti-Human FGFR2 (13042-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.8 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 13042-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

13042-1-PBS bindet in IHC, IF/ICC, FC (Intra), Indirect ELISA FGFR2/CD332 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: FGFR2/CD332 fusion protein Ag3701
• Vollständiger Name: fibroblast growth factor receptor 2
• Berechnetes Molekulargewicht: 709 aa, 79 kDa
• GenBank-Zugangsnummer: BC039243
• Gene symbol: FGFR2
• Gene ID (NCBI): 2263
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

FGFR2 (Fibroblast growth factor receptor 2) is a tyrosine-protein kinase that acts as a cell-surface receptor for fibroblast growth factors and plays an essential role in the regulation of cell proliferation, differentiation, migration, and apoptosis. Ligand binding leads to the activation of several signaling pathways, such as RAS, MAPK1/ERK2, MAPK3/ERK1, and the MAP Kinase signaling pathway, as well as the AKT1 signaling pathway. Mutations in the gene of FGFR2 are associated with Crouzon syndrome, Pfeiffer syndrome, Craniosynostosis, Apert syndrome, Jackson-Weiss syndrome, Beare-Stevenson cutis gyrata syndrome, Saethre-Chotzen syndrome, and syndromic craniosynostosis. This polyclonal antibody raised against 358-704aa of human FGFR2 can cross-react with other members of the FGFR family.