FGFR2/CD332 Rekombinanter Antikörper

FGFR2/CD332 Rekombinant Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

241383G9

Kat-Nr. : 84205-4-PBS

Synonyme

FGFR2, 241383G9, BEK, BFR 1, CD332

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  WB analysis using 84205-4-RR (same clone as 84205-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84205-4-RR (FGFR2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84205-4-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse liver using 84205-4-RR (same clone as 84205-4-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 84205-4-RR (FGFR2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84205-4-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84205-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84205-4-RR against Human FGFR2 were performed. The affinity constant is 18.8 nM.

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Geprüfte Anwendungen

Produktinformation

84205-4-PBS bindet in WB, IHC, Indirect ELISA FGFR2/CD332 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: FGFR2/CD332 fusion protein Eg1265
• Vollständiger Name: fibroblast growth factor receptor 2
• Berechnetes Molekulargewicht: 92kd
• Beobachtetes Molekulargewicht: 85 kDa
• GenBank-Zugangsnummer: NM_000141.4
• Gene symbol: FGFR2
• Gene ID (NCBI): 2263
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

FGFR2 (Fibroblast growth factor receptor 2) is a tyrosine-protein kinase that acts as a cell-surface receptor for fibroblast growth factors and plays an essential role in the regulation of cell proliferation, differentiation, migration, and apoptosis. Ligand binding leads to the activation of several signaling pathways, such as RAS, MAPK1/ERK2, MAPK3/ERK1, and the MAP Kinase signaling pathway, as well as the AKT1 signaling pathway. Mutations in the gene of FGFR2 are associated with Crouzon syndrome, Pfeiffer syndrome, Craniosynostosis, Apert syndrome, Jackson-Weiss syndrome, Beare-Stevenson cutis gyrata syndrome, Saethre-Chotzen syndrome, and syndromic craniosynostosis.