FGFR2/CD332 Rekombinanter Antikörper

FGFR2/CD332 Rekombinant Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

CloneNo.

241383G9

Rezensionen (1)

Kat-Nr. : 84205-4-RR

Synonyme

FGFR2, 241383G9, BEK, BFR 1, CD332

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  WB analysis using 84205-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84205-4-RR (FGFR2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse liver using 84205-4-RR

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 84205-4-RR (FGFR2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  Affinity and Kinetic Characterization of 84205-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84205-4-RR against Human FGFR2 were performed. The affinity constant is 18.8 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HCT 116-Zellen, HepG2-Zellen
• Erfolgreiche Detektion in IHC: Mauslebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

84205-4-RR bindet in WB, IHC, ELISA FGFR2/CD332 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: FGFR2/CD332 fusion protein Eg1265
• Vollständiger Name: fibroblast growth factor receptor 2
• Berechnetes Molekulargewicht: 92kd
• Beobachtetes Molekulargewicht: 85 kDa
• GenBank-Zugangsnummer: NM_000141.4
• Gene symbol: FGFR2
• Gene ID (NCBI): 2263
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

FGFR2 (Fibroblast growth factor receptor 2) is a tyrosine-protein kinase that acts as a cell-surface receptor for fibroblast growth factors and plays an essential role in the regulation of cell proliferation, differentiation, migration, and apoptosis. Ligand binding leads to the activation of several signaling pathways, such as RAS, MAPK1/ERK2, MAPK3/ERK1, and the MAP Kinase signaling pathway, as well as the AKT1 signaling pathway. Mutations in the gene of FGFR2 are associated with Crouzon syndrome, Pfeiffer syndrome, Craniosynostosis, Apert syndrome, Jackson-Weiss syndrome, Beare-Stevenson cutis gyrata syndrome, Saethre-Chotzen syndrome, and syndromic craniosynostosis.

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Reyes (Verified Customer) (09-30-2025)

FGFR2 (in green) worked well marking the axons of the neurons in human FFPE tissue

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Human Brain Cortex

FGFR2/CD332 Antibody Immunofluorescence validation (1:100 dilution) in Human Brain Cortex (Cat no:84205-4-RR)