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FGFR4 Polyklonaler Antikörper

FGFR4 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 11098-1-AP

Synonyme

CD334, FGFR 4, FGFR4, JTK2, TKF

Galerie der Validierungsdaten

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  • Western Blot (WB) analysis of HepG2 cells using FGFR4 Polyclonal antibody (11098-1-AP)

    WB analysis of HepG2 using 11098-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 11098-1-AP (FGFR4 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse lung tissue using FGFR4 Polyclonal antibody (11098-1-AP)

    WB analysis of mouse lung using 11098-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 11098-1-AP (FGFR4 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using FGFR4 Polyclonal antibody (11098-1-AP)

    IHC staining of human breast cancer using 11098-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11098-1-AP (FGFR4 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using FGFR4 Polyclonal antibody (11098-1-AP)

    IHC staining of human breast cancer using 11098-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11098-1-AP (FGFR4 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using FGFR4 Polyclonal antibody (11098-1-AP)

    IF Staining of HepG2 using 11098-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 11098-1-AP (FGFR4 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Geprüfte Anwendungen

Erfolgreiche Detektion in WBHepG2-Zellen, Mauslungengewebe
Erfolgreiche Detektion in IHChumanes Mammakarzinomgewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
Erfolgreiche Detektion in IFHepG2-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:200-1:1000
Immunhistochemie (IHC)IHC : 1:50-1:500
Immunfluoreszenz (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

11098-1-AP bindet in WB, IHC, IF, ELISA FGFR4 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
In Publikationen genannte Reaktivitäthuman, Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen FGFR4 fusion protein Ag1573
Vollständiger Name fibroblast growth factor receptor 4
Berechnetes Molekulargewicht 88 kDa
Beobachtetes Molekulargewicht 100-110 kDa
GenBank-ZugangsnummerBC011847
Gene symbol FGFR4
Gene ID (NCBI) 2264
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Protokolle

Produktspezifische Protokolle
WB protocol for FGFR4 antibody 11098-1-APProtokoll herunterladen
IHC protocol for FGFR4 antibody 11098-1-APProtokoll herunterladen
IF protocol for FGFR4 antibody 11098-1-APProtokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
mouseWB

Biochem Pharmacol

Association between the perturbation of bile acid homeostasis and valproic acid-induced hepatotoxicity.

Authors - Yanan Chen
View Article
mouseWB

Biomed Pharmacother

Bupleuri radix extract ameliorates impaired lipid metabolism in high-fat diet-induced obese mice via gut microbia-mediated regulation of FGF21 signaling pathway.

Authors - Liucen Wu
View Article
human

J Cell Biochem

Glycosylation of FGFR4 in cholangiocarcinoma regulates receptor processing and cancer signaling.

Authors - Andrew J Phillips
View Article
humanIHC, WB

Int J Oncol

Role of fibroblast growth factor 4 in the growth and metastasis of colorectal cancer.

Authors - Yanwei Ye
View Article
humanIHC

Cancer Res Treat

FGFR4 Gly388Arg Polymorphism Affects the Progression of Gastric Cancer by Activating STAT3 Pathway to Induce Epithelial to Mesenchymal Transition.

Authors - Yanwei Ye
View Article
mouseIF

Invest Ophthalmol Vis Sci

Characterization of a Spontaneously Immortalized Murine Müller Glial Cell Line QMMuC-1.

Authors - Josy Augustine
View Article