FGFR4 Polyklonaler Antikörper

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• KD/KO Validated

FGFR4 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(19)

Rezensionen (1)

Kat-Nr. : 11098-1-AP

Synonyme

CD334, EC:2.7.10.1, FGFR 4, FGFR-4, JTK2

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  WB analysis using 11098-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11098-1-AP (FGFR4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 11098-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11098-1-AP (FGFR4 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 11098-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11098-1-AP (FGFR4 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 11098-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 11098-1-AP (FGFR4 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: L02-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 11 publications below
• IHC: See 4 publications below
• IF: See 7 publications below

Produktinformation

11098-1-AP bindet in WB, IHC, IF/ICC, ELISA FGFR4 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: FGFR4 fusion protein Ag1573
• Vollständiger Name: fibroblast growth factor receptor 4
• Berechnetes Molekulargewicht: 88 kDa
• Beobachtetes Molekulargewicht: 95, 100-110 kDa
• GenBank-Zugangsnummer: BC011847
• Gene symbol: FGFR4
• Gene ID (NCBI): 2264
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Fibroblast growth factor receptor 4 (FGFR4) is a member of a highly conserved tyrosine kinase family, along with FGFR1-3. This family consists of an intracellular tyrosine kinase domain, a single transmembrane domain, and extracellular ligand-binding domains (PMID: 32492514). FGFR4 is the predominant FGFR isoform present in human hepatocytes. FGFR4 has been proposed to play a role in the observed induction of hepatocyte proliferation and carcinogenesis by FGF19; however, contradicting evidence proposing a protective role for FGFR4 in suppressing hepatoma progression has also been proposed(PMID: 20018895). While the role of FGFR4 in cancer remains to be fully elucidated, several findings suggest that this receptor may be an important player in Hepatocellular carcinoma (HCC) development and/or progression(PMID: 10336501).

Publikationen

Species	Application	Title
mouse,human	WB,IHC	J Med Chem; FGF19-Based Mini Probe Targeting FGFR4 for Diagnosis and Surgical Navigation of Hepatocellular Carcinoma; Authors - Jiaxian Cai ; KD Validated
mouse	WB	Biochem Pharmacol; Association between the perturbation of bile acid homeostasis and valproic acid-induced hepatotoxicity.; Authors - Yanan Chen
mouse	WB	Biomed Pharmacother; Bupleuri radix extract ameliorates impaired lipid metabolism in high-fat diet-induced obese mice via gut microbia-mediated regulation of FGF21 signaling pathway.; Authors - Liucen Wu
human		J Cell Biochem; Glycosylation of FGFR4 in cholangiocarcinoma regulates receptor processing and cancer signaling.; Authors - Andrew J Phillips
human	WB,IHC	Int J Oncol; Role of fibroblast growth factor 4 in the growth and metastasis of colorectal cancer.; Authors - Yanwei Ye
human	IHC	Cancer Res Treat; FGFR4 Gly388Arg Polymorphism Affects the Progression of Gastric Cancer by Activating STAT3 Pathway to Induce Epithelial to Mesenchymal Transition.; Authors - Yanwei Ye

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Jillian (Verified Customer) (05-27-2025)

Overall we weren't as happy with the antibody as we wanted to be. There appeared to be a lot of nonspecific binding in the NHP samples that we tested it on

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1:400
• Cell Tissue Type: Liver, Frontal, Temporal