FGFR4 Polyklonaler Antikörper

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• KD/KO Validated

FGFR4 Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 11098-1-PBS

Synonyme

CD334, EC:2.7.10.1, FGFR 4, FGFR-4, JTK2

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  WB analysis using 11098-1-AP (same clone as 11098-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 11098-1-AP (FGFR4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11098-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 11098-1-AP (same clone as 11098-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11098-1-AP (FGFR4 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11098-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 11098-1-AP (same clone as 11098-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11098-1-AP (FGFR4 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11098-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 11098-1-AP (same clone as 11098-1-PBS)

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 11098-1-AP (FGFR4 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 11098-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

11098-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA FGFR4 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: FGFR4 fusion protein Ag1573
• Vollständiger Name: fibroblast growth factor receptor 4
• Berechnetes Molekulargewicht: 88 kDa
• Beobachtetes Molekulargewicht: 95, 100-110 kDa
• GenBank-Zugangsnummer: BC011847
• Gene symbol: FGFR4
• Gene ID (NCBI): 2264
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Fibroblast growth factor receptor 4 (FGFR4) is a member of a highly conserved tyrosine kinase family, along with FGFR1-3. This family consists of an intracellular tyrosine kinase domain, a single transmembrane domain, and extracellular ligand-binding domains (PMID: 32492514). FGFR4 is the predominant FGFR isoform present in human hepatocytes. FGFR4 has been proposed to play a role in the observed induction of hepatocyte proliferation and carcinogenesis by FGF19; however, contradicting evidence proposing a protective role for FGFR4 in suppressing hepatoma progression has also been proposed(PMID: 20018895). While the role of FGFR4 in cancer remains to be fully elucidated, several findings suggest that this receptor may be an important player in Hepatocellular carcinoma (HCC) development and/or progression(PMID: 10336501).