Fibrinogen Gamma Chain Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

Fibrinogen Gamma Chain Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

15

Kat-Nr. : 15841-1-AP

Synonyme

FGG, fibrinogen gamma chain

---

Galerie der Validierungsdaten

• 

  WB analysis of human spleen using 15841-1-AP

  human spleen tissue were subjected to SDS PAGE followed by western blot with 15841-1-AP (Fibrinogen gamma chain antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 15841-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15841-1-AP (Fibrinogen Gamma Chain antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 15841-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 15841-1-AP (Fibrinogen gamma chain antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human hepatocirrhosis using 15841-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 15841-1-AP (Fibrinogen gamma chain antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human hepatocirrhosis using 15841-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 15841-1-AP (Fibrinogen gamma chain antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, humanes Lebergewebe, humanes Milzgewebe, Mauslebergewebe, Mausmilzgewebe, Rattenmilzgewebe
• Erfolgreiche Detektion in IHC: humanes Leberzirrhosegewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 6 publications below
• IHC: See 6 publications below
• IF: See 6 publications below

Produktinformation

15841-1-AP bindet in WB, IHC, IF, ELISA Fibrinogen Gamma Chain und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Fibrinogen Gamma Chain fusion protein Ag8597
• Vollständiger Name: fibrinogen gamma chain
• Berechnetes Molekulargewicht: 453aa,52 kDa; 437aa,50 kDa
• Beobachtetes Molekulargewicht: 52 kDa
• GenBank-Zugangsnummer: BC007044
• Gene symbol: FGG
• Gene ID (NCBI): 2266
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Fibrinogen is a soluble plasma glycoprotein synthesized in the liver. It is composed of two sets of three structurally different subunits: alpha (FGA), beta (FGB), gamma (FGG). Fibrinogen is converted by thrombin into fibrin during blood coagulation. Fibrinogen and fibrin play overlapping roles in blood clotting, fibrinolysis, cellular and matrix interactions, the inflammatory response, wound healing, and neoplasia (PMID: 16102057). FGG is the gamma chain of fibrinogen. Mutations in the gene of FGG lead to several disorders, including dysfibrinogenemia, hypofibrinogenemia and thrombophilia.

Publikationen

Species	Application	Title
human,rat	IHC,IF	Blood; Fibrinogen β-derived Bβ(15-42) peptide protects against kidney ischemia/reperfusion injury.; Authors - Krishnamoorthy Aparna A
mouse		J Thromb Haemost; Chronic liver injury drives non-traditional intrahepatic fibrin(ogen) cross-linking via tissue transglutaminase.; Authors - L G Poole
human	WB	Sci Rep; C-reactive protein (CRP) recognizes uric acid crystals and recruits proteases C1 and MASP1.; Authors - Anika Alberts
rat	IHC	J Neurotrauma; FTY720 Attenuates Neuropathic Pain after Spinal Cord Injury by Decreasing Systemic and Local Inflammation in a Rat Spinal Cord Compression Model.; Authors - Kazuyoshi Yamazaki
human	WB,IHC,IF	Front Neurosci; Fibrinogen Chains Intrinsic to the Brain.; Authors - Eugene V Golanov ; KD Validated
mouse	IHC	Cell Mol Neurobiol; Myelin Debris Impairs Tight Junctions and Promotes the Migration of Microvascular Endothelial Cells in the Injured Spinal Cord.; Authors - Fei Yao

Verwandte Produkte

Fibrinogen Gamma Chain Monoclonal antibody

66158-1-Ig

Fibrinogen Beta Chain Polyclonal antibody

16747-1-AP

Fibrinogen Beta Chain Monoclonal antibody

66186-1-Ig

CD41/Integrin Alpha 2B Polyclonal antibody

24552-1-AP

CD61 / Integrin Beta 3 Polyclonal antibody

18309-1-AP

F2 Polyclonal antibody

24295-1-AP