Fibrinogen Gamma Chain Monoklonaler Antikörper

Fibrinogen Gamma Chain Monoklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgM

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

CloneNo.

2B2B8

Kat-Nr. : 66158-1-Ig

Synonyme

FGG, 2B2B8, PRO2061

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  WB analysis of L02 using 66158-1-Ig

  L02 cells were subjected to SDS PAGE followed by western blot with 66158-1-Ig (Fibrinogen gamma chain antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human peripheral blood leukocyte using 66158-1-Ig

  human peripheral blood leukocyte were subjected to SDS PAGE followed by western blot with 66158-1-Ig (Fibrinogen Gamma Chain antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human peripheral blood platelets using 66158-1-Ig

  human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 66158-1-Ig (Fibrinogen Gamma Chain antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 66158-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66158-1-Ig (Fibrinogen Gamma Chain antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 66158-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66158-1-Ig (Fibrinogen Gamma Chain antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 66158-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66158-1-Ig (Fibrinogen gamma chain antibody at dilution of 1:50.

• 

  IHC staining of human kidney using 66158-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66158-1-Ig (Fibrinogen gamma chain antibody at dilution of 1:50.

• 

  IF Staining of L02 using 66158-1-Ig

  Immunofluorescent analysis of L02 cells using 66158-1-Ig (Fibrinogen gamma chain antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: human peripheral blood leukocyte, L02-Zellen
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe, humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: L02-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:1000-1:4000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IHC: See 1 publications below

Produktinformation

66158-1-Ig bindet in WB, IHC, IF/ICC, ELISA Fibrinogen Gamma Chain und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgM
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Fibrinogen Gamma Chain fusion protein Ag8817
• Vollständiger Name: fibrinogen gamma chain
• Berechnetes Molekulargewicht: 453aa,52 kDa; 437aa,50 kDa
• Beobachtetes Molekulargewicht: 52 kDa
• GenBank-Zugangsnummer: BC007044
• Gene symbol: Fibrinogen Gamma Chain
• Gene ID (NCBI): 2266
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Thiophiler Affinitätschromatograph
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Fibrinogen is a soluble plasma glycoprotein synthesized in the liver. It is composed of two sets of three structurally different subunits: alpha (FGA), beta (FGB), gamma (FGG). Fibrinogen is converted by thrombin into fibrin during blood coagulation. Fibrinogen and fibrin play overlapping roles in blood clotting, fibrinolysis, cellular and matrix interactions, the inflammatory response, wound healing, and neoplasia (PMID: 16102057). FGG is the gamma chain of fibrinogen. Mutations in the gene of FGG lead to several disorders, including dysfibrinogenemia, hypofibrinogenemia and thrombophilia.

Publikationen

Species	Application	Title
human	IHC	Front Genet; Elevated Serum FGG Levels Prognosticate and Promote the Disease Progression in Prostate Cancer.; Authors - H H Peng
		Mol Ther Nucleic Acids; Antithrombotic Effect of shRNA Target F12 Mediated by Adeno-Associated Virus.; Authors - Fanfan Li