FGL1 Polyklonaler Antikörper

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• KD/KO Validated

FGL1 Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

19

Kat-Nr. : 16000-1-AP

Synonyme

FGL1, fibrinogen like 1, Fibrinogen like protein 1, Hepassocin, HFREP 1, HFREP1, HP 041, HPS, LFIRE 1, LFIRE1

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Galerie der Validierungsdaten

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  WB analysis using 16000-1-AP

  Human placenta tissue were subjected to SDS PAGE followed by western blot with 16000-1-AP (FGL1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 16000-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 16000-1-AP (FGL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 16000-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16000-1-AP (FGL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse thymus using 16000-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 16000-1-AP (FGL1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 16000-1-AP

  IP Result of anti-FGL1 (IP:16000-1-AP, 4ug; Detection:16000-1-AP 1:500) with HeLa cells lysate 2000ug.

• 

  IHC staining of human liver cancer using 16000-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16000-1-AP (FGL1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 16000-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16000-1-AP (FGL1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 16000-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 16000-1-AP (FGL1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 16000-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 16000-1-AP (FGL1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human hepatocirrhosis using 16000-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 16000-1-AP (FGL1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human hepatocirrhosis using 16000-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 16000-1-AP (FGL1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Plazenta-Gewebe, HepG2-Zellen, humanes Lebergewebe, Maus-Thymusgewebe
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe, humanes Leberzirrhosegewebe, Mauslebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 16 publications below
• IHC: See 11 publications below
• IF: See 2 publications below

Produktinformation

16000-1-AP bindet in WB, IP, IHC, IF, ELISA FGL1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: FGL1 fusion protein Ag8581
• Vollständiger Name: fibrinogen-like 1
• Berechnetes Molekulargewicht: 312 aa, 36 kDa
• Beobachtetes Molekulargewicht: 34 kDa, 68 kDa
• GenBank-Zugangsnummer: BC007047
• Gene symbol: FGL1
• Gene ID (NCBI): 2267
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

FGL1 is a 68 kDa protein that comprises two disulfide linked 34 kDa homodimers. FGL1 is a predominantly liver expressed protein that has been implicated as both a hepatoprotectant and a hepatocyte mitogen. FGL1 expression is decreased in hepatocellular carcinoma (HCC), and it may play a role in the development of hepatocellular carcinomas.

Publikationen

Species	Application	Title
human	WB,IHC	Transl Res; Role of placental fibrinogen-like protein 1 in gestational diabetes.; Authors - Lin Kang
mouse	IHC	Front Immunol; Fibrinogen-like protein 1 promotes liver-resident memory T-cell exhaustion in hepatocellular carcinoma; Authors - Changjie Yang
mouse	WB	Diabetologia; PMID: 27221093; Authors - Hung-Tsung Wu ; KD Validated
mouse	WB,IHC	J Nanobiotechnology; Regulating the immunosuppressive tumor microenvironment to enhance breast cancer immunotherapy using pH-responsive hybrid membrane-coated nanoparticles.; Authors - Chunai Gong
human	WB	Int J Mol Sci; Fibrinogen-Like Protein 1 Modulates Sorafenib Resistance in Human Hepatocellular Carcinoma Cells.; Authors - Yeonghoon Son
human,mouse	WB	J Clin Endocrinol Metab; The Hepatic Protection Effects of Hepassocin in Hyperglycemic Crisis.; Authors - Horng-Yih Ou