FH Monoklonaler Antikörper

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FH Monoklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

Hausschwein, human, Kaninchen, Maus, Ratte

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1A9C8

Kat-Nr. : 68162-1-PBS

Synonyme

HsFH, Fumarase, EC:4.2.1.2, 1A9C8

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  WB analysis using 68162-1-Ig (same clone as 68162-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68162-1-Ig (FH antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68162-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 68162-1-Ig (same clone as 68162-1-PBS)

  WB result of FH antibody (68162-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FH transfected HepG2 cells. This data was developed using the same antibody clone with 68162-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 68162-1-Ig (same clone as 68162-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 68162-1-Ig (FH antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68162-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse cerebellum using 68162-1-Ig (same clone as 68162-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 68162-1-Ig (FH antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68162-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 68162-1-Ig (same clone as 68162-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 68162-1-Ig (FH antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68162-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

68162-1-PBS bindet in WB, IHC, Indirect ELISA FH und zeigt Reaktivität mit Hausschwein, human, Kaninchen, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Kaninchen, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: FH fusion protein Ag30254
• Vollständiger Name: fumarate hydratase
• Berechnetes Molekulargewicht: 510 aa, 55 kDa
• Beobachtetes Molekulargewicht: 45-50 kDa
• GenBank-Zugangsnummer: BC003108
• Gene symbol: FH
• Gene ID (NCBI): 2271
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Fumarate hydratase, or fumarase, is an enzymatic component of the tricarboxylic acid cycle, catalyzing the conversion of fumarate to malate. It belongs to the class-II fumarase/aspartase family. This protein has 2 isoforms produced by alternative initiation.