FH Rekombinanter Antikörper

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FH Rekombinant Antikörper für WB, IHC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IP, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

3K16

Kat-Nr. : 82508-1-PBS

Synonyme

3K16, EC:4.2.1.2, Fumarase, HsFH

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  WB analysis using 82508-1-RR (same clone as 82508-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82508-1-RR (FH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82508-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 82508-1-RR (same clone as 82508-1-PBS)

  WB result of FH antibody (82508-1-RR; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FH transfected HepG2 cells. This data was developed using the same antibody clone with 82508-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse liver using 82508-1-RR (same clone as 82508-1-PBS)

  IP result of anti-FH (IP:82508-1-RR, 4ug; Detection:82508-1-RR 1:4000) with mouse liver tissue lysate 1840 ug. This data was developed using the same antibody clone with 82508-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach using 82508-1-RR (same clone as 82508-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 82508-1-RR (FH antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82508-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 82508-1-RR (same clone as 82508-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 82508-1-RR (FH antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82508-1-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 82508-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82508-1-RR against Human FH were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

Produktinformation

82508-1-PBS bindet in WB, IHC, IP, Indirect ELISA FH und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: FH fusion protein Ag1934
• Vollständiger Name: fumarate hydratase
• Berechnetes Molekulargewicht: 510 aa, 55 kDa
• Beobachtetes Molekulargewicht: 45 kDa
• GenBank-Zugangsnummer: BC003108
• Gene symbol: FH
• Gene ID (NCBI): 2271
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

FH(fumarate hydratase) belongs to the class-II fumarase/aspartase family. It is an enzymatic component of the tricarboxylic acid cycle, catalyzing the conversion of fumarate to malate. FH also acts as a tumor suppressor function of FH in sporadic kidney cancer(PMID:21695080). Mutations in this gene can cause fumarase deficiency and lead to progressive encephalopathy.