FHIT Rekombinanter Antikörper

FHIT Rekombinant Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

CloneNo.

240564D9

Kat-Nr. : 83710-1-PBS

Synonyme

AP3Aase, AP3A hydrolase, Adenylylsulfate-ammonia adenylyltransferase, Adenylylsulfatase, Adenosine 5'-monophosphoramidase FHIT

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  WB analysis using 83710-1-RR (same clone as 83710-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83710-1-RR (FHIT antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83710-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 83710-1-RR (same clone as 83710-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 83710-1-RR (FHIT antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83710-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83710-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83710-1-RR against Human FHIT were performed. The affinity constant is 5.01 nM.

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Geprüfte Anwendungen

Produktinformation

83710-1-PBS bindet in WB, IHC, ELISA FHIT und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: FHIT fusion protein Ag4181
• Vollständiger Name: fragile histidine triad gene
• Berechnetes Molekulargewicht: 147 aa, 17 kDa
• Beobachtetes Molekulargewicht: 15-17 kDa
• GenBank-Zugangsnummer: BC032336
• Gene symbol: FHIT
• Gene ID (NCBI): 2272
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The candidate tumor suppressor gene, FHIT(Fragile histidine triad protein), encompasses the common human chromosomal fragile site at 3p14.2, the hereditary renal cancer translocation breakpoint, and cancer cell homozygous deletions. Fhit hydrolyzes dinucleotide 5',5'''-P1,P3-triphosphate in vitro and mutation of a central histidine abolishes hydrolase activity(PMID:9391102). FHIT has a major role in regulating beta-catenin-mediated gene transcription.