FHL2 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

FHL2 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(15)

Rezensionen (1)

Kat-Nr. : 21619-1-AP

Synonyme

AAG11, DRAL, FHL 2, FHL-2, LIM domain protein DRAL

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  WB analysis using 21619-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21619-1-AP (FHL2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse skeletal muscle using 21619-1-AP

  IP result of anti-FHL2 (IP:21619-1-AP, 4ug; Detection:21619-1-AP 1:1000) with mouse skeletal muscle tissue lysate 2200ug.

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  IHC staining of human cervical cancer using 21619-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 21619-1-AP (FHL2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 21619-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 21619-1-AP (FHL2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human skin cancer using 21619-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 21619-1-AP (FHL2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human skin cancer using 21619-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 21619-1-AP (FHL2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 21619-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 21619-1-AP (FHL2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 21619-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 21619-1-AP (FHL2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse ovary using 21619-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 21619-1-AP (FHL2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse ovary using 21619-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 21619-1-AP (FHL2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat heart using 21619-1-AP

  Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 21619-1-AP (FHL2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat ovary using 21619-1-AP

  Immunohistochemical analysis of paraffin-embedded rat ovary tissue slide using 21619-1-AP (FHL2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat ovary using 21619-1-AP

  Immunohistochemical analysis of paraffin-embedded rat ovary tissue slide using 21619-1-AP (FHL2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat heart using 21619-1-AP

  Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 21619-1-AP (FHL2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 21619-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 21619-1-AP (FHL2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of A431 using 21619-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using FHL2 antibody (21619-1-AP) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, hTERT-RPE1-Zellen, NIH/3T3-Zellen, U2OS-Zellen
• Erfolgreiche IP: Maus-Skelettmuskelgewebe
• Erfolgreiche Detektion in IHC: humanes Zervixkarzinomgewebe, humanes Hautkrebsgewebe, Mausnierengewebe, Maus-Eierstockgewebe, Rattenherzgewebe, Ratten-Eierstockgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen, A431-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:1000-1:4000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 15 publications below
• IHC: See 4 publications below
• IF: See 4 publications below
• IP: See 1 publications below
• CoIP: See 4 publications below

Produktinformation

21619-1-AP bindet in WB, IHC, IF/ICC, IP, CoIP, ELISA FHL2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: FHL2 fusion protein Ag1378
• Vollständiger Name: four and a half LIM domains 2
• Berechnetes Molekulargewicht: 32 kDa
• Beobachtetes Molekulargewicht: 32 kDa
• GenBank-Zugangsnummer: BC014397
• Gene symbol: FHL2
• Gene ID (NCBI): 2274
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

FHL2(Four and a half LIM domains protein 2), also known as DRAL. It is located in cytoplasm and nucleus. The protein is expressed in skeletal muscle and heart. May function as a molecular transmitter linking various signaling pathways to transcriptional regulation. Negatively regulates the transcriptional repressor E4F1 and may function in cell growth. Inhibits the transcriptional activity of FOXO1 and its apoptotic function by enhancing the interaction of FOXO1 with SIRT1 and FOXO1 deacetylation. Negatively regulates the calcineurin/NFAT signaling pathway in cardiomyocytes (PMID: 28717008). The molecular weight of FHL2 is 32 kDa.

Publikationen

Species	Application	Title
human	WB	Nat Commun; A signature motif in LIM proteins mediates binding to checkpoint proteins and increases tumour radiosensitivity.; Authors - Xiaojie Xu
human	WB,IF,CoIP	EBioMedicine; Up-regulated FHL2 inhibits ovulation through interacting with androgen receptor and ERK1/2 in polycystic ovary syndrome.; Authors - Ruiqiong Zhou
mouse,human	WB,IHC,IF,CoIP	Cell Death Dis; FHL2 deficiency impairs follicular development and fertility by attenuating EGF/EGFR/YAP signaling in ovarian granulosa cells; Authors - Chen Wang ; KD Validated
mouse	WB,CoIP	iScience; Genetic ablation of diabetes-associated gene Ccdc92 reduces obesity and insulin resistance in mice; Authors - Lu Ren
human	WB	Int J Mol Sci; FHL2 Inhibits SARS-CoV-2 Replication by Enhancing IFN-β Expression through Regulating IRF-3; Authors - Zhiqiang Xu ; KD Validated
human	WB,IHC	J Virol; Structural and Functional Characterization of Host FHL1 Protein Interaction with Hypervariable Domain of Chikungunya Virus nsP3 Protein.; Authors - Tetyana Lukash

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Marina (Verified Customer) (03-24-2025)

80 micrograms of cell lysate (U-373 MG cells). Primary antibody dilution 1:1000, overnight incubation at 4 degrees.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Glioblastoma cell line

FHL2 Antibody Western Blot validation (1:1000 dilution) in Glioblastoma cell line (Cat no:21619-1-AP)