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FHL2 Rekombinanter Antikörper

FHL2 Rekombinant Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

250585E1

Kat-Nr. : 86060-1-PBS

Synonyme

AAG11, DRAL, FHL 2, FHL-2, LIM domain protein DRAL

Filter:
  • Western Blot (WB) analysis of various lysates using FHL2 Recombinant antibody (86060-1-RR)

    WB analysis using 86060-1-RR (same clone as 86060-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86060-1-RR (FHL2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86060-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using FHL2 Recombinant antibody (86060-1-RR)

    IF Staining of HeLa using 86060-1-RR (same clone as 86060-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using FHL2 antibody (86060-1-RR, Clone: 250585E1 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 86060-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

86060-1-PBS bindet in WB, IF/ICC, Indirect ELISA FHL2 und zeigt Reaktivität mit human, Maus

Getestete Reaktivität human, Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen FHL2 fusion protein Ag29655
Vollständiger Name four and a half LIM domains 2
Berechnetes Molekulargewicht 32 kDa
Beobachtetes Molekulargewicht30-32 kDa
GenBank-ZugangsnummerBC014397
Gene symbol FHL2
Gene ID (NCBI) 2274
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

FHL2(Four and a half LIM domains protein 2), also known as DRAL. It is located in cytoplasm and nucleus. The protein is expressed in skeletal muscle and heart. May function as a molecular transmitter linking various signaling pathways to transcriptional regulation. Negatively regulates the transcriptional repressor E4F1 and may function in cell growth. Inhibits the transcriptional activity of FOXO1 and its apoptotic function by enhancing the interaction of FOXO1 with SIRT1 and FOXO1 deacetylation. Negatively regulates the calcineurin/NFAT signaling pathway in cardiomyocytes (PMID: 28717008). The molecular weight of FHL2 is 32 kDa.