FHL3 Polyklonaler Antikörper

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• KD/KO Validated

FHL3 Polyklonal Antikörper für IHC, IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

12

Kat-Nr. : 11028-2-AP

Synonyme

FHL 3, FHL3, four and a half LIM domains 3, Skeletal muscle LIM protein 2, SLIM 2, SLIM2

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Galerie der Validierungsdaten

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  WB analysis of K-562 using 11028-2-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 11028-2-AP (FHL3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse ovary using 11028-2-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 11028-2-AP (FHL3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of K-562 using 11028-2-AP

  IP Result of anti-FHL3 (IP:11028-2-AP, 4ug; Detection:11028-2-AP 1:500) with K-562 cells lysate 1200ug.

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  IHC staining of human ovary tumor using 11028-2-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 11028-2-AP (FHL3 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human ovary tumor using 11028-2-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 11028-2-AP (FHL3 antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: K-562-Zellen, Maus-Eierstockgewebe
• Erfolgreiche IP: K-562-Zellen
• Erfolgreiche Detektion in IHC: humanes Ovarialkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 10 publications below
• IHC: See 3 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Produktinformation

11028-2-AP bindet in WB, IP, IHC, IF, ELISA FHL3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: FHL3 fusion protein Ag1495
• Vollständiger Name: four and a half LIM domains 3
• Berechnetes Molekulargewicht: 31 kDa
• Beobachtetes Molekulargewicht: 31 kDa
• GenBank-Zugangsnummer: BC011697
• Gene symbol: FHL3
• Gene ID (NCBI): 2275
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Four and a half LIM domain (FHL) protein 3 is a member of the FHL protein family that has roles in the regulation of signal transduction, survival, cell adhesion, and mobility. It also involves in the development and progression of liver cancer. The FHL proteins have been shown to regulate a variety of transcription factors, including SMAD proteins, β-catenin, SRF, AP-1, NFAT, FOXO1, MyoD, and the androgen receptor. Beside, the FHL proteins may involve in muscle growth and differentiation. Recent research revealed FHL3 was a PCBP2 target, also involved in the growth and induced apoptosis of glioma cell (23585479). This antibody recognizes specifically the 34kd FHL3 protein.

Publikationen

Species	Application	Title
human	WB,IHC	J Clin Invest; RNA-binding protein PCBP2 modulates glioma growth by regulating FHL3.; Authors - Han Wei W
human	WB	Nat Commun; A signature motif in LIM proteins mediates binding to checkpoint proteins and increases tumour radiosensitivity.; Authors - Xiaojie Xu
human	WB,IP	Cell Death Differ; FHL3 links cell growth and self-renewal by modulating SOX4 in glioma.; Authors - Wei Han ; KD Validated
human	WB,IHC,IF	Aging (Albany NY); FHL3 promotes pancreatic cancer invasion and metastasis through preventing the ubiquitination degradation of EMT associated transcription factors.; Authors - Pengping Li ; KD Validated
human	WB	J Virol; The four and a half LIM family members are novel interactants of the human T-cell leukemia virus type 1 Tax oncoprotein.; Authors - McCabe áine á
human	IF	Front Oncol; FHL3 Contributes to EMT and Chemotherapy Resistance Through Up-Regulation of Slug and Activation of TGFβ/Smad-Independent Pathways in Gastric Cancer.; Authors - Guodong Cao