FIP1L1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

FIP1L1 Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 12276-1-PBS

Synonyme

FIP1, FIP1-like 1 protein, Pre-mRNA 3'-end-processing factor FIP1, RHE

---

• 

  WB analysis of K-562 using 12276-1-AP (same clone as 12276-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 12276-1-AP (FIP1L1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12276-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 12276-1-AP (same clone as 12276-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 12276-1-AP (FIP1L1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12276-1-PBS in a different storage buffer formulation.

• 

  WB analysis of MCF-7 using 12276-1-AP (same clone as 12276-1-PBS)

  WB result of FIP1L1 antibody (12276-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FIP1L1 transfected MCF-7 cells. This data was developed using the same antibody clone with 12276-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary tumor using 12276-1-AP (same clone as 12276-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 12276-1-AP (FIP1L1 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 12276-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 12276-1-AP (same clone as 12276-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12276-1-AP (FIP1L1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12276-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 12276-1-AP (same clone as 12276-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12276-1-AP (FIP1L1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12276-1-PBS in a different storage buffer formulation.

• 

  IF Staining of MCF-7 using 12276-1-AP (same clone as 12276-1-PBS)

  Immunofluorescent analysis of MCF-7 cells, using FIP1L1 antibody 12276-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 12276-1-PBS in a different storage buffer formulation.

• 

  IF Staining of MCF-7 using 12276-1-AP (same clone as 12276-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using FIP1L1 antibody (12276-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 12276-1-PBS in a different storage buffer formulation.

---

Geprüfte Anwendungen

Produktinformation

12276-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA FIP1L1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: FIP1L1 fusion protein Ag2921
• Vollständiger Name: FIP1 like 1 (S. cerevisiae)
• Berechnetes Molekulargewicht: 594 aa, 66 kDa
• Beobachtetes Molekulargewicht: 67 kDa
• GenBank-Zugangsnummer: BC017724
• Gene symbol: FIP1L1
• Gene ID (NCBI): 81608
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

FIP1L1, also known as FIP1, encodes a subunit of the CPSF (cleavage and polyadenylation specificity factor) complex that polyadenylates the 3' end of mRNA precursors. The FIP1L1-PDGFRA fusion gene encodes a constitutively activated tyrosine kinase that links the first 233 amino acids of FIP1L1 to the last 523 amino acids of PDGFRA. This gene fusion and chromosomal deletion causes some forms of idiopathic hypereosinophilic syndrome (HES)( PMID: 12660384).