FIP1L1 Rekombinanter Antikörper

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• KD/KO Validated

FIP1L1 Rekombinant Antikörper für WB, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, FC (Intra), ELISA

Konjugation

Unkonjugiert

CloneNo.

240658C4

Kat-Nr. : 83863-2-RR

Synonyme

240658C4, FIP1, FIP1-like 1 protein, Pre-mRNA 3'-end-processing factor FIP1, RHE

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  WB analysis of MCF-7 using 83863-2-RR

  WB result of FIP1L1 antibody (83863-2-RR; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FIP1L1 transfected MCF-7 cells.

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  WB analysis using 83863-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83863-2-RR (FIP1L1 antibody) at dilution of 1:7500 incubated at room temperature for 1.5 hours.

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  FC experiment of A549 using 83863-2-RR

  1x10^6 A549 cells were intracellularly stained with 0.25 ug FIP1L1 Recombinant antibody (83863-2-RR, Clone:240658C4) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

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  FC experiment of U-2 OS using 83863-2-RR

  1x10^6 U-2 OS were intracellularly stained with 0.25 ug FIP1L1 Recombinant antibody (83863-2-RR, Clone:240658C4) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

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  Affinity and Kinetic Characterization of 83863-2-RR

  Biolayer interferometry (BLl) kinetic assays of 83863-2-RR against Human FIP1L1 were performed. The affinity constant is 10.8 pM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HEK-293T-Zellen, MCF-7-Zellen, Maus-Thymusgewebe, U-251-Zellen
• Erfolgreiche Detektion in FC (Intra): A549-Zellen, U2OS-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:15000
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

83863-2-RR bindet in WB, FC (Intra), ELISA FIP1L1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: FIP1L1 fusion protein Ag2921
• Vollständiger Name: FIP1 like 1 (S. cerevisiae)
• Berechnetes Molekulargewicht: 594 aa, 66 kDa
• Beobachtetes Molekulargewicht: 67-80 kDa
• GenBank-Zugangsnummer: BC017724
• Gene symbol: FIP1L1
• Gene ID (NCBI): 81608
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

FIP1L1, also known as FIP1, encodes a subunit of the CPSF (cleavage and polyadenylation specificity factor) complex that polyadenylates the 3' end of mRNA precursors. The FIP1L1-PDGFRA fusion gene encodes a constitutively activated tyrosine kinase that links the first 233 amino acids of FIP1L1 to the last 523 amino acids of PDGFRA. This gene fusion and chromosomal deletion causes some forms of idiopathic hypereosinophilic syndrome (HES)( PMID: 12660384).