FIP1L1 Rekombinanter Antikörper

FIP1L1 Rekombinant Antikörper für IHC, IF/ICC, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

IHC, IF/ICC, FC (Intra), ELISA

Konjugation

Unkonjugiert

CloneNo.

240658A8

Kat-Nr. : 83863-3-RR

Synonyme

240658A8, FIP1, FIP1-like 1 protein, Pre-mRNA 3'-end-processing factor FIP1, RHE

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  IHC staining of human ovary cancer using 83863-3-RR

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83863-3-RR (FIP1L1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of MCF-7 using 83863-3-RR

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using FIP1L1 antibody (83863-3-RR, Clone: 240658A8 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  FC experiment of A549 using 83863-3-RR

  1x10^6 A549 cells were intracellularly stained with 0.25 ug FIP1L1 Recombinant antibody (83863-3-RR, Clone:240658A8) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

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  FC experiment of U-2 OS using 83863-3-RR

  1x10^6 U-2 OS were intracellularly stained with 0.25 ug FIP1L1 Recombinant antibody (83863-3-RR, Clone:240658A8) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

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  Affinity and Kinetic Characterization of 83863-3-RR

  Biolayer interferometry (BLl) kinetic assays of 83863-3-RR against Human FIP1L1 were performed. The affinity constant is 1.63 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: human ovary cancer tissue; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: MCF-7-Zellen
• Erfolgreiche Detektion in FC (Intra): A549-Zellen, U2OS-Zellen

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:250-1:1000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:125-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

83863-3-RR bindet in IHC, IF/ICC, FC (Intra), ELISA FIP1L1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: FIP1L1 fusion protein Ag2921
• Vollständiger Name: FIP1 like 1 (S. cerevisiae)
• Berechnetes Molekulargewicht: 594 aa, 66 kDa
• GenBank-Zugangsnummer: BC017724
• Gene symbol: FIP1L1
• Gene ID (NCBI): 81608
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

FIP1L1, also known as FIP1, encodes a subunit of the CPSF (cleavage and polyadenylation specificity factor) complex that polyadenylates the 3' end of mRNA precursors. The FIP1L1-PDGFRA fusion gene encodes a constitutively activated tyrosine kinase that links the first 233 amino acids of FIP1L1 to the last 523 amino acids of PDGFRA. This gene fusion and chromosomal deletion causes some forms of idiopathic hypereosinophilic syndrome (HES)( PMID: 12660384).