FITC Monoklonaler Antikörper

FITC Monoklonal Antikörper für WB, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

FITC und mehr (1)

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

CloneNo.

3C10A1

Kat-Nr. : 68132-1-Ig

Synonyme

Fluorescein isothiocyanate

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  WB analysis using 68132-1-Ig

  WB result of FITC antibody (68132-1-Ig; 1:40000; incubated at room temperature for 1.5 hours) with FITC conjugated protein and un-conjugated protein as negative control. Antibody to TDP-43 as the positive control to target protein.

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  WB analysis of BSA using 68132-1-Ig

  WB results of FITC antibody (68132-1-Ig, 1:10000) with BSA and FITC conjugated BSA.

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  ELISA experiment of FITC conjugated antibody using 68132-1-Ig

  Indirect ELISA and competitive ELISA results show that this antibody is specific to FITC. Indirect ELISA was performed by coating FITC conjugated BSA at 0ng/well followed by blocking with 1% BSA. Serial diluted primary antibody was added to the plates and incubated at 37℃. HRP-goat anti-mouse was used for detection (red curve, refer to top X-right Y axis). Competitive ELISA was performed similarly except that different concentration of FITC (active group been quenched) was mixed in 3.3ng/mL primary antibody (blue curve, refere to bottom X-left Y axis).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: FITC-TDP43, bsa
• Erfolgreiche Detektion in ELISA: FITC conjugated antibody

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Enzyme-linked Immunosorbent Assay (ELISA): ELISA :

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

68132-1-Ig bindet in WB, ELISA FITC und zeigt Reaktivität mit FITC

• Getestete Reaktivität: FITC
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: FITC
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Fluorescein isothiocyanate (FITC) is a derivative of fluorescein used in wide-ranging applications. It is the original fluorescein molecule functionalized with an isothiocyanate reactive group (-N=C=S), replacing a hydrogen atom on the bottom ring of the structure. It is typically available as a mixture of isomers, fluorescein 5-isothiocyanate (5-FITC) and fluorescein 6-isothiocyanate (6-FITC). FITC is reactive towards nucleophiles including amine and sulfhydryl groups on proteins. CAS number of FITC: 27072-45-3 (mixture); 3326-32-7, 5-isomer; 18861-78-4 (6-isomer). This mouse monoclonal antibody detects the FITC only. This antibody can be coated in the plate to capture the FITC conjugated protein or antibody.

Publikationen

Species	Application	Title
mouse	WB	Environ Health Perspect; Organophosphorus Flame Retardants and Metabolic Disruption: An in Silico, in Vitro, and in Vivo Study Focusing on Adiponectin Receptors; Authors - Ying Liu